- データシート
- 使用論文
製品の概要
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製品名Sodium salicylate
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製品の詳細Cell-permeable anti-inflammatory agent
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生理活性の詳細Cell-permeable anti-inflammatory agent. Inhibits cyclooxygenases preventing prostaglandin formation. Inhibits components of the MAPK cascade, NF-κB and AP-1. Induces apoptosis through p38 MAPK activation.
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精製度> 99%
製品の特性
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体系名2-Hydroxybenzoic acid sodium salt
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分子量160.10
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構造式
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分子式C7H5NaO3
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CAS 番号54-21-7
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保存方法Store at Room Temperature. Store under desiccating conditions. The product can be stored for up to 12 months.
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溶解性Soluble in water to 100 mM
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使用に関する注意
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
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由来
Synthetic
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研究分野
アプリケーション
Our Abpromise guarantee covers the use of ab120746 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Functional Studies | Use at an assay dependent concentration. |
画像
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Functional Studies - Sodium salicylate (ab120746)THP1 cells were incubated at 37°C for 40h with vehicle control (0 µM) and different concentrations of sodium salicylate (ab120746). Decreased expression of scavenging receptor SR-BI (ab52629) in THP1 cells correlates with an increase in sodium salicylate concentration, as described in literature.
Whole cell lysates were prepared with RIPA buffer (containing protease inhibitors and sodium orthovanadate), 10 µg of each were loaded on the gel and the WB was run under reducing conditions. After transfer the membrane was blocked for an hour using 5% BSA before being incubated with ab52629 at 1/2000 dilution and ab8227 at 1 µg/ml overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP (ab97051) at 1/10000 dilution and visualised using ECL development solution.
参考文献
This product has been referenced in:
- Amann R & Peskar BA Anti-inflammatory effects of aspirin and sodium salicylate. Eur J Pharmacol 447:1-9 (2002). Read more (PubMed: 12106797) »
- Schwenger P et al. Activation of p38 mitogen-activated protein kinase by sodium salicylate leads to inhibition of tumor necrosis factor-induced IkappaB alpha phosphorylation and degradation. Mol Cell Biol 18:78-84 (1998). Read more (PubMed: 9418855) »
- Mitchell JA et al. Sodium salicylate inhibits cyclo-oxygenase-2 activity independently of transcription factor (nuclear factor kappaB) activation: role of arachidonic acid. Mol Pharmacol 51:907-12 (1997). Read more (PubMed: 9187256) »
- Schwenger P et al. Inhibition of tumor necrosis factor-induced p42/p44 mitogen-activated protein kinase activation by sodium salicylate. J Biol Chem 271:8089-94 (1996). Read more (PubMed: 8626494) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE, NOT FOR USE IN HUMANS"