製品の概要

  • 製品名Anti-Sodium Potassium ATPase antibody
    Sodium Potassium ATPase 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Sodium Potassium ATPase
  • 特異性ab58475 detects endogenous levels of total Sodium Potassium ATPase protein.
  • アプリケーション適用あり: IHC-Fr, ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).

  • ポジティブ・コントロール
    • Human brain tissue and 293 cell extracts treated with PMA (125ng/ml, 30mins).

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab58475 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr
ELISA
IHC-P
WB
  • 追加情報ELISA: 1/5000.
    IHC-Fr: Use at an assay dependent dilution (PMID 19923323).
    IHC-P: 1/50 - 1/100.
    WB: 1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
    • 配列類似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
    • 翻訳後修飾Phosphorylation on Tyr-10 modulates pumping activity.
    • 細胞内局在Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATPase Na+/K+ transporting alpha antibody
      • adenosinetriphosphatase antibody
      • AT1A1_HUMAN antibody
      • ATP1A1 antibody
      • ATP1A4 antibody
      • ATP1AL2 antibody
      • ATP1B antibody
      • ATP1B1 antibody
      • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
      • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
      • ATPase Na+/K+ transporting beta 1 polypeptide antibody
      • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
      • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
      • Beta 1-subunit of Na(+),K(+)-ATPase antibody
      • Na(+)/K(+) ATPase alpha-1 subunit antibody
      • Na(+)/K(+) ATPase alpha-4 subunit antibody
      • Na+, K+ ATPase alpha subunit antibody
      • Na+/K+ ATPase 1 antibody
      • Na+/K+ ATPase 4 antibody
      • Na+/K+ ATPase, alpha-D polypeptide antibody
      • Na, K-ATPase beta-1 polypeptide antibody
      • Na, K-ATPase, alpha-A catalytic polypeptide antibody
      • Na,K-ATPase catalytic subunit alpha-A protein antibody
      • Na,K-ATPase subunit alpha-C antibody
      • polypeptide-like 2 antibody
      • Sodium pump 1 antibody
      • sodium pump 4 antibody
      • Sodium pump subunit alpha-1 antibody
      • sodium pump subunit alpha-4 antibody
      • sodium pump subunit beta-1 antibody
      • sodium-potassium ATPase catalytic subunit alpha-1 antibody
      • sodium-potassium ATPase catalytic subunit alpha-4 antibody
      • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
      • sodium-potassium ATPase, alpha 4 polypeptide antibody
      • sodium-potassium-ATPase, alpha 1 polypeptide antibody
      • Sodium/potassium transporting ATPase alpha 1 chain antibody
      • Sodium/potassium transporting ATPase subunit beta 1 antibody
      • sodium/potassium-dependent ATPase beta-1 subunit antibody
      • sodium/potassium-transporting ATPase alpha-4 chain antibody
      • sodium/potassium-transporting ATPase beta-1 chain antibody
      • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
      • sodium/potassium-transporting ATPase subunit alpha-4 antibody
      see all

    Anti-Sodium Potassium ATPase antibody 画像

    • All lanes : Anti-Sodium Potassium ATPase antibody (ab58475) at 1/500 dilution

      Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
      Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide


      Predicted band size : 113 kDa
      Observed band size : 113 kDa
    • ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.

    Anti-Sodium Potassium ATPase antibody (ab58475) 使用論文

    This product has been referenced in:
    • Lovisa S  et al. Epithelial-to-mesenchymal transition induces cell cycle arrest and parenchymal damage in renal fibrosis. Nat Med 21:998-1009 (2015). IHC-P ; Mouse . Read more (PubMed: 26236991) »
    • Leung Y  et al. Label Retaining Cells (LRCs) with Myoepithelial Characteristic from the Proximal Acinar Region Define Stem Cells in the Sweat Gland. PLoS One 8:e74174 (2013). Read more (PubMed: 24058524) »

    See all 4 Publications for this product

    Product Wall

    The stock solution of the Optiblot DTT Reducer (10X) ab119199 in 2M. This means that once diluted 10 times this produces a 200 mM solution. Therefore if you usually use 10 mM with your samples I would suggest diluting this 200 times. The higher concent...

    Read More

    Thank you for contacting us.   I have looked through our catalog of products searching for membrane marker that may perform better for you in adipose tissue. I concur with your assessment that an Anti-pan Cadherin antibody may be a better choice an...

    Read More

    Thank you for your enquiry. This antibody will detect the alpha 1 subunit and not the beta 1 subunit. As far as we are aware, cross reactivity with monkey has not yet been tested for use with ab58475. All species cross-reactivity information is ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"