Anti-Sodium Potassium ATPase 抗体 - Plasma Membrane Marker (ab58475)
Key features and details
- Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker
Sodium Potassium ATPase 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Sodium Potassium ATPase - Plasma Membrane Marker -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic non-phosphopeptide derived from human Sodium Potassium ATPase around the phosphorylation site of serine 16 (A-V-SP-E-H).
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg+2 and Ca+2 -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab58475の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/50 - 1/100.
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WB |
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa).
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特記事項 |
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IHC-P
1/50 - 1/100. |
WB
1/500 - 1/1000. Detects a band of approximately 113 kDa (predicted molecular weight: 113 kDa). |
ターゲット情報
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機能
This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients. -
配列類似性
Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily. -
翻訳後修飾
Phosphorylation on Tyr-10 modulates pumping activity. -
細胞内局在
Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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参照データベース
- Entrez Gene: 476 Human
- Entrez Gene: 480 Human
- Entrez Gene: 481 Human
- Omim: 182310 Human
- Omim: 182330 Human
- Omim: 607321 Human
- SwissProt: P05023 Human
- SwissProt: P05026 Human
see all -
別名
- ATPase Na+/K+ transporting alpha antibody
- adenosinetriphosphatase antibody
- AT1A1_HUMAN antibody
see all
画像
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All lanes : Anti-Sodium Potassium ATPase antibody - Plasma Membrane Marker (ab58475) at 1/500 dilution
Lane 1 : 293 cell extracts treated with PMA (125ng/ml, 30mins)
Lane 2 : 293 cell extracts treated with PMA (125ng/ml, 30mins) with immunising peptide
Predicted band size: 113 kDa
Observed band size: 113 kDa -
ab58475 at 1/50 dilution staining Sodium Potassium ATPase in human brain by Immunohistochemistry, Paraffin embedded tissue, in the absence or presence of the immunising peptide.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (24)
ab58475 は 24 報の論文で使用されています。
- Bhattacharya I et al. Plasma Membrane-Derived Liposomes Exhibit Robust Antiviral Activity against HSV-1. Viruses 14:N/A (2022). PubMed: 35458528
- Mandriani B et al. Development of anti-somatostatin receptors CAR T cells for treatment of neuroendocrine tumors. J Immunother Cancer 10:N/A (2022). PubMed: 35764366
- Kobuke K et al. ATP1A1 Mutant in Aldosterone-Producing Adenoma Leads to Cell Proliferation. Int J Mol Sci 22:N/A (2021). PubMed: 34681640
- Li L & Zhang C Venlafaxine Attenuated the Cognitive and Memory Deficit in Mice Exposed to Isoflurane Alone. Front Neurol 12:591223 (2021). PubMed: 33708168
- Keshri GK et al. Effects of Pulsed 810?nm Al-Ga-As Diode Laser on Wound Healing Under Immunosuppression: A Molecular Insight. Lasers Surg Med 52:424-436 (2020). PubMed: 31483061