製品の概要

  • 製品名Anti-Sodium Potassium ATPase antibody
    Sodium Potassium ATPase 一次抗体 製品一覧
  • 製品の詳細
    Chicken polyclonal to Sodium Potassium ATPase
  • 特異性Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Chicken Serum as well as purified and partially purified ATPase, Sodium, Potassium [Canine Kidney].
  • アプリケーション適用あり: ICC/IF, IHC-Fr, ELISA, WBmore details
  • 種交差性
    交差種: Dog
  • 免疫原

    ATPase, Sodium, Potassium [Canine Kidney]

  • ポジティブ・コントロール
    • ICC: MDCK cells ELISA: 1ug recombinant protein
  • 特記事項Centrifuge product if not completely clear after standing at room temperature. This product is for research use only and is not intended for therapeutic or diagnostic applications. Please note that we have 2 concentrations of this antibody: 1mg/ml and 10mg/ml. Please check the vial label.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab353 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/500.

(see review). Use amplification.

IHC-Fr 1/100.

(see review)

ELISA 1/1000 - 1/6000.

This product has been assayed against 1.0 ug of ATPase, Sodium, Potassium in a standard sandwich ELISA using Peroxidase conjugated Affinity Purified anti-Chicken IgG [H&L] (Rabbit)and ABTS (2,2’-azino-bis-[3-ethylbenthia-zoline-6-sulfonic acid])as a substrate for 30 minutes at room temperature.

WB 1/100 - 1/500.

ターゲット情報

  • 機能This is the catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of sodium and potassium ions across the plasma membrane. This action creates the electrochemical gradient of sodium and potassium ions, providing the energy for active transport of various nutrients.
  • 配列類似性Belongs to the cation transport ATPase (P-type) (TC 3.A.3) family. Type IIC subfamily.
  • 翻訳後修飾Phosphorylation on Tyr-10 modulates pumping activity.
  • 細胞内局在Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
  • Information by UniProt
  • 別名
    • ATPase Na+/K+ transporting alpha antibody
    • adenosinetriphosphatase antibody
    • AT1A1_HUMAN antibody
    • ATP1A1 antibody
    • ATP1A4 antibody
    • ATP1AL2 antibody
    • ATP1B antibody
    • ATP1B1 antibody
    • ATPase Na+/K+ transporting alpha 1 polypeptide antibody
    • ATPase Na+/K+ transporting alpha 4 polypeptide antibody
    • ATPase Na+/K+ transporting beta 1 polypeptide antibody
    • ATPase, Na+/K+ transporting, alpha polypeptide-like 2 antibody
    • ATPase, Na+/K+ transporting, beta 1 polypeptide antibody
    • Beta 1-subunit of Na(+),K(+)-ATPase antibody
    • Na(+)/K(+) ATPase alpha-1 subunit antibody
    • Na(+)/K(+) ATPase alpha-4 subunit antibody
    • Na+, K+ ATPase alpha subunit antibody
    • Na+/K+ ATPase 1 antibody
    • Na+/K+ ATPase 4 antibody
    • Na+/K+ ATPase, alpha-D polypeptide antibody
    • Na, K-ATPase beta-1 polypeptide antibody
    • Na, K-ATPase, alpha-A catalytic polypeptide antibody
    • Na,K-ATPase catalytic subunit alpha-A protein antibody
    • Na,K-ATPase subunit alpha-C antibody
    • polypeptide-like 2 antibody
    • Sodium pump 1 antibody
    • sodium pump 4 antibody
    • Sodium pump subunit alpha-1 antibody
    • sodium pump subunit alpha-4 antibody
    • sodium pump subunit beta-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-1 antibody
    • sodium-potassium ATPase catalytic subunit alpha-4 antibody
    • sodium-potassium ATPase subunit beta 1 (non-catalytic) antibody
    • sodium-potassium ATPase, alpha 4 polypeptide antibody
    • sodium-potassium-ATPase, alpha 1 polypeptide antibody
    • Sodium/potassium transporting ATPase alpha 1 chain antibody
    • Sodium/potassium transporting ATPase subunit beta 1 antibody
    • sodium/potassium-dependent ATPase beta-1 subunit antibody
    • sodium/potassium-transporting ATPase alpha-4 chain antibody
    • sodium/potassium-transporting ATPase beta-1 chain antibody
    • Sodium/potassium-transporting ATPase subunit alpha-1 antibody
    • sodium/potassium-transporting ATPase subunit alpha-4 antibody
    see all

Anti-Sodium Potassium ATPase antibody 画像

  • ab353 staining Sodium Potassium ATPase in Madin-Darby Canine Kidney (MDCK) cells by Immunocytochemistry/ Immunofluorescence.
    Cells were fixed in methanol/acetone (1:1) at -20°C for 10 minutes, permeabilized with 0.5% Triton X-100 in PBS, and blocked with 5% BSA in PBS for 1 hour before incubation with ab353 at a 1/250 dilution. An Alexa Fluor–conjugated secondary antibody was used at a dilution of 1/500 – 1/1000.

Anti-Sodium Potassium ATPase antibody (ab353) 使用論文

This product has been referenced in:
  • Braune EB  et al. S/T phosphorylation of DLL1 is required for full ligand activity in vitro but dispensable for DLL1 function in vivo during embryonic patterning and marginal zone B cell development. Mol Cell Biol 34:1221-33 (2014). Read more (PubMed: 24449764) »
  • Liu R  et al. Itraconazole suppresses the growth of glioblastoma through induction of autophagy: involvement of abnormal cholesterol trafficking. Autophagy 10:1241-55 (2014). Read more (PubMed: 24905460) »

See all 7 Publications for this product

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Merci de nous avoir contactés.

Après avoir effectué une recherche pour un anticorps marqueur de la membrane plasmique qui pourrait convenir à vos besoins (autre qu'un anticorps de lapin ou IgG1 de souris),je n'ai tr...

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Thank you for your enquiry. The epitope has not been mapped in the cases of ab7671 and ab8344 therefore we do not know which part of the protein is recognised by those antibodies. As the immunogen used to generate ab353 is purified Na,K-ATPase from...

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This antibody was found to be reactive against the intact enzyme and enzyme subunits (alpha at 100 kDa and beta at 50 kDa) as well as glycosylated and non-glycosylated enzyme forms by western blot. The product is also reactive against 1.0ug of ATPase, ...

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Reactivity with other mammalian sources is unknown, but likely, due to the nature of the polyclonal antibody.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"