Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) 抗体 [14D5] (ab72729)

製品の概要

  • 製品名Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5]
    Sodium / Hydrogen Exchanger 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [14D5] to Sodium / Hydrogen Exchanger 3 (phospho S552)
  • アプリケーション適用あり: WB, IP, ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human, Opossum
  • 免疫原

    Synthetic phospho-peptide(rat) made from residues surrounding Serine 552.

  • ポジティブ・コントロール
    • human kidney lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファーPreservative: 0.05% Sodium Azide
    Constituents: Tris glycine, 150mM Sodium chloride
  • Concentration information loading...
  • 精製度Protein G purified
  • ポリ/モノモノクローナル
  • クローン名14D5
  • アイソタイプIgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 93 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/50 - 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • 配列類似性Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • 翻訳後修飾Phosphorylated by PKA, which inhibits activity.
  • 細胞内局在Membrane. In intestinal epithelial cells, localizes to the ileal brush border.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC126718 antibody
    • MGC126720 antibody
    • Na(+)/H(+) exchanger 3 antibody
    • NHE 3 antibody
    • NHE-3 antibody
    • NHE3 antibody
    • SL9A3_HUMAN antibody
    • SLC9A 3 antibody
    • Slc9a3 antibody
    • Sodium/hydrogen exchanger 3 antibody
    • Sodium/hydrogen exchanger, apical epithelial antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), member 3 antibody
    • Solute carrier family 9 member 3 antibody
    see all

Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] 画像

  • Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] (ab72729) at 5 µg/ml + human kidney lysate

    Predicted band size : 93 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] (ab72729) 使用論文

ab72729 has not yet been referenced specifically in any publications.

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Thank you for your enquiry.

The antibody has been tested in Western Blot using Human, Mouse, and Rat Colon, Small Intestine, and Kidney samples. There were bands expressed around 25kDa and 50kDa, with weaker bands at approx. 80-90 kDa observ...

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Thank you for your enquiry. These antibodies are phosphospecific and will not cross react with the non-phospho antigen. They will only detect the whole protein if it is phosphorylated at the repective serine residues. I hope this information hel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"