Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) 抗体 [14D5] (ab72729)

製品の概要

  • 製品名
    Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5]
    Sodium / Hydrogen Exchanger 3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [14D5] to Sodium / Hydrogen Exchanger 3 (phospho S552)
  • アプリケーション
    適用あり: WB, IP, ELISA, ICC/IF, IHC-P, IHC-Frmore details
  • 種交差性
    交差種: Mouse, Rat, Rabbit, Human, Opossum
  • 免疫原

    Synthetic phospho-peptide(rat) made from residues surrounding Serine 552.

  • ポジティブ・コントロール
    • human kidney lysate
  • 特記事項

    Abcam is committed to meeting high standards of ethical manufacturing and has decided to discontinue this product by June 2019 as it has been generated by the ascites method. We are sorry for any inconvenience this may cause.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • バッファー
    Preservative: 0.05% Sodium Azide
    Constituents: Tris glycine, 150mM Sodium chloride
  • Concentration information loading...
  • 精製度
    Protein G purified
  • ポリ/モノ
    モノクローナル
  • クローン名
    14D5
  • アイソタイプ
    IgG2a
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab72729 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 93 kDa).
IP Use at an assay dependent concentration.
ELISA Use at an assay dependent concentration.
ICC/IF 1/50 - 1/1000.
IHC-P Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction.
  • 配列類似性
    Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family.
  • 翻訳後修飾
    Phosphorylated by PKA, which inhibits activity.
  • 細胞内局在
    Membrane. In intestinal epithelial cells, localizes to the ileal brush border.
  • Information by UniProt
  • 参照データベース
  • 別名
    • MGC126718 antibody
    • MGC126720 antibody
    • Na(+)/H(+) exchanger 3 antibody
    • NHE 3 antibody
    • NHE-3 antibody
    • NHE3 antibody
    • SL9A3_HUMAN antibody
    • SLC9A 3 antibody
    • Slc9a3 antibody
    • Sodium/hydrogen exchanger 3 antibody
    • Sodium/hydrogen exchanger, apical epithelial antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), isoform 3 antibody
    • Solute carrier family 9 (sodium/hydrogen exchanger), member 3 antibody
    • Solute carrier family 9 member 3 antibody
    see all

画像

  • Anti-Sodium / Hydrogen Exchanger 3 (phospho S552) antibody [14D5] (ab72729) at 5 µg/ml + human kidney lysate

    Predicted band size : 93 kDa
    Observed band size : 85 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 55 kDa. We are unsure as to the identity of these extra bands.

参考文献

ab72729 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for your enquiry.

The antibody has been tested in Western Blot using Human, Mouse, and Rat Colon, Small Intestine, and Kidney samples. There were bands expressed around 25kDa and 50kDa, with weaker bands at approx. 80-90 kDa observ...

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Thank you for your enquiry. These antibodies are phosphospecific and will not cross react with the non-phospho antigen. They will only detect the whole protein if it is phosphorylated at the repective serine residues. I hope this information hel...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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