Anti-Sodium/Hydrogen Exchanger 3/NHE-3 抗体 (ab95299)
Key features and details
- Rabbit polyclonal to Sodium/Hydrogen Exchanger 3/NHE-3
- Suitable for: WB, IHC-P
- Reacts with: Rat
- Isotype: IgG
製品の概要
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製品名
Anti-Sodium/Hydrogen Exchanger 3/NHE-3 antibody
Sodium/Hydrogen Exchanger 3/NHE-3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Sodium/Hydrogen Exchanger 3/NHE-3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Rat -
免疫原
Synthetic peptide corresponding to Rat Sodium/Hydrogen Exchanger 3/NHE-3 aa 809-831 (C terminal).
Sequence:DSFLQADGPEEQLQPASPESTHM
Database link: NP_036786 -
ポジティブ・コントロール
- Rat kidney tissue lysate. IHC-P: rat colon tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
Preservative: 0.09% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab95299の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 93 kDa).
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IHC-P |
1/25.
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特記事項 |
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WB
1/1000. Detects a band of approximately 84 kDa (predicted molecular weight: 93 kDa). |
IHC-P
1/25. |
ターゲット情報
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機能
Involved in pH regulation to eliminate acids generated by active metabolism or to counter adverse environmental conditions. Major proton extruding system driven by the inward sodium ion chemical gradient. Plays an important role in signal transduction. -
配列類似性
Belongs to the monovalent cation:proton antiporter 1 (CPA1) transporter (TC 2.A.36) family. -
翻訳後修飾
Phosphorylated by PKA, which inhibits activity. -
細胞内局在
Membrane. In intestinal epithelial cells, localizes to the ileal brush border. - Information by UniProt
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参照データベース
- Entrez Gene: 24784 Rat
- SwissProt: P26433 Rat
- Unigene: 9706 Rat
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別名
- MGC126718 antibody
- MGC126720 antibody
- Na(+)/H(+) exchanger 3 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded formalin fixed (4%) rat colon tissue labelling Exchanger 3/NHE-3 with ab95299 at 1/25 dilution, followed by secondary antibody Goat Anti-Rabbit IgG (Alexa Fluor 488) (Green). Membrane and secretory staining on rat colon tissue is observed. Counter stained with Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alex Fluor 633 (Red) F-Actin stain. (C) Exchanger 3/NHE-3 Antibody (D) Composite image.
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Western blot detection of Sodium/Hydrogen Exchanger 3 / NHE-3 in Rat kidney tissues using ab95299 at 1:1000 dilution.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab95299 は 5 報の論文で使用されています。
- Zarei K et al. Anion Transport Across Human Gallbladder Organoids and Monolayers. Front Physiol 13:882525 (2022). PubMed: 35685290
- Wang JL et al. Expression, Localization, and Effect of High Salt Intake on Electroneutral Na+/HCO3- Cotransporter NBCn2 in Rat Small Intestine: Implication in Intestinal NaCl Absorption. Front Physiol 10:1334 (2019). PubMed: 31736772
- Tingskov SJ et al. Tamoxifen Decreases Lithium-Induced Natriuresis in Rats With Nephrogenic Diabetes Insipidus. Front Physiol 9:903 (2018). PubMed: 30050465
- Wang YY et al. SLC9A3 Protein Is Critical for Acrosomal Formation in Postmeiotic Male Germ Cells. Int J Mol Sci 19:N/A (2017). PubMed: 29286340
- Wang YY et al. Loss of SLC9A3 decreases CFTR protein and causes obstructed azoospermia in mice. PLoS Genet 13:e1006715 (2017). IHC-P ; Mouse . PubMed: 28384194