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RabMAb

Anti-SOD2/MnSOD 抗体 [EPR2560Y] (ab68155)

製品の概要

  • 製品名
    Anti-SOD2/MnSOD antibody [EPR2560Y]
    SOD2/MnSOD 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EPR2560Y] to SOD2/MnSOD
  • アプリケーション
    適用あり: IHC-Fr, WB, IHC-Pmore details
    適用なし: Flow Cyt,ICC or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    corresponding to Human SOD2/MnSOD aa 1-100 (N terminal).

  • ポジティブ・コントロール
    • WB: Rat brain lysate and SH-SY5Y lysate. IHC-P: Human liver tissue sections.
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab68155 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-Fr Use at an assay dependent concentration. PubMed: 21906276
WB 1/1000 - 1/2000. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P 1/100 - 1/250.
  • 追加情報
    Is unsuitable for Flow Cyt,ICC or IP.
  • ターゲット情報

    • 機能
      Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
    • 関連疾患
      Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
    • 配列類似性
      Belongs to the iron/manganese superoxide dismutase family.
    • 翻訳後修飾
      Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
    • 細胞内局在
      Mitochondrion matrix.
    • Information by UniProt
    • 参照データベース
    • 別名
      • Indophenoloxidase B antibody
      • IPO B antibody
      • IPOB antibody
      • Manganese containing superoxide dismutase antibody
      • Manganese SOD antibody
      • Manganese superoxide dismutase antibody
      • Mangano superoxide dismutase antibody
      • Mn SOD antibody
      • Mn superoxide dismutase antibody
      • MNSOD antibody
      • MVCD6 antibody
      • SOD 2 antibody
      • SOD2 antibody
      • SODM_HUMAN antibody
      • Superoxide dismutase [Mn] mitochondrial antibody
      • Superoxide dismutase [Mn], mitochondrial antibody
      • Superoxide dismutase 2 mitochondrial antibody
      see all

    Anti-SOD2/MnSOD antibody [EPR2560Y] 画像



    • Performed under reducing conditions.

      Predicted band size : 25 kDa

      Lane 1: Wild-type HAP1 cell lysate (20 µg)
      Lane 2: SOD2 knockout HAP1 cell lysate (20 µg)
      Lane 3: Human heart tissue lysate (20 µg)
      Lane 4: Rat heart tissue lysate (20 µg)
      Lanes 1 - 4: Merged signal (red and green). Green - ab68155 observed at 29 kDa. Red - loading control, ab8245, observed at 37 kDa.

      ab68155 was shown to specifically react with SOD2 when SOD2 knockout samples were used. Wild-type and SOD2 knockout samples were subjected to SDS-PAGE. ab68155 and ab8245 (loading control to GAPDH) were diluted at 1/1000 and 1/10 000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed ab216776 secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.

    • All lanes : Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) at 1/500 dilution

      Lane 1 : Rat brain lysate
      Lane 2 : SH-SY5Y lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size : 25 kDa
      Observed band size : 25 kDa
    • ab68155 at 1:100 dilution staining Superoxide Dismutase 2 in human liver tissue sections.
    • Immunohistochemical analysis of frozen rat lung tissue taken from rats with monocrotaline-exposure/pneumonectomy, staining SOD2 with ab68155.

    Anti-SOD2/MnSOD antibody [EPR2560Y] (ab68155) 使用論文

    This product has been referenced in:
    • Cheng XY  et al. AMP-activated protein kinase reduces inflammatory responses and cellular senescence in pulmonary emphysema. Oncotarget 8:22513-22523 (2017). WB ; Mouse . Read more (PubMed: 28186975) »
    • Wang L  et al. Effects of 17ß-estradiol and 2-methoxyestradiol on the oxidative stress-hypoxia inducible factor-1 pathway in hypoxic pulmonary hypertensive rats. Exp Ther Med 13:2537-2543 (2017). Read more (PubMed: 28565876) »

    See all 8 Publications for this product

    Product Wall

    Application
    Western blot
    Loading amount
    40 µg
    Gel Running Conditions
    Reduced Denaturing (12% tris-glycine gel)
    Sample
    Human Tissue lysate - whole (heart)
    Specification
    heart
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Oct 23 2014

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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