製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SOD2/MnSOD
  • 特異性
    ab118340 is expected to recognize both reported isoforms (NP_000627.2; NP_001019637.1). The immunizing peptide represents the acetylation site including K122 according to isoform A.
  • アプリケーション
    適用あり: ELISA, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog, Pig, Zebrafish
  • 免疫原

    Synthetic peptide corresponding to Human SOD2/MnSOD aa 119-130 (internal sequence) (Cysteine residue).
    Sequence:

    EAIKRDFGSFDK


    Database link: P04179

  • ポジティブ・コントロール
    • Mouse and Rat spinal cord and Human Brain (cerebellum) lysates.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab118340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA Use at an assay dependent concentration.

Antibody detection limit dilution 1:64,000.

WB Use a concentration of 0.01 - 0.1 µg/ml. Detects a band of approximately 24 kDa (predicted molecular weight: 25 kDa).

ターゲット情報

  • 機能
    Destroys superoxide anion radicals which are normally produced within the cells and which are toxic to biological systems.
  • 関連疾患
    Genetic variation in SOD2 is associated with susceptibility to microvascular complications of diabetes type 6 (MVCD6) [MIM:612634]. These are pathological conditions that develop in numerous tissues and organs as a consequence of diabetes mellitus. They include diabetic retinopathy, diabetic nephropathy leading to end-stage renal disease, and diabetic neuropathy. Diabetic retinopathy remains the major cause of new-onset blindness among diabetic adults. It is characterized by vascular permeability and increased tissue ischemia and angiogenesis.
  • 配列類似性
    Belongs to the iron/manganese superoxide dismutase family.
  • 翻訳後修飾
    Nitrated under oxidative stress. Nitration coupled with oxidation inhibits the catalytic activity.
  • 細胞内局在
    Mitochondrion matrix.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Indophenoloxidase B antibody
    • IPO B antibody
    • IPOB antibody
    • Manganese containing superoxide dismutase antibody
    • Manganese SOD antibody
    • Manganese superoxide dismutase antibody
    • Mangano superoxide dismutase antibody
    • Mn SOD antibody
    • Mn superoxide dismutase antibody
    • MNSOD antibody
    • MVCD6 antibody
    • SOD 2 antibody
    • SOD2 antibody
    • SODM_HUMAN antibody
    • Superoxide dismutase [Mn] mitochondrial antibody
    • Superoxide dismutase [Mn], mitochondrial antibody
    • Superoxide dismutase 2 mitochondrial antibody
    see all

Anti-SOD2/MnSOD antibody 画像

  • All lanes : Anti-SOD2/MnSOD antibody (ab118340)

    Lane 1 : Human Cerebellum lysate
    Lane 2 : Mouse Brain lysate
    Lane 3 : Rat Brain lysate
    Lane 4 : Pig Brain lysate

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 25 kDa
  • All lanes : Anti-SOD2/MnSOD antibody (ab118340)

    Lane 1 : HeLa cell lysate
    Lane 2 : HepG2
    Lane 3 : HEK293
    Lane 4 : NIH3T3

    Lysates/proteins at 35 µg per lane.


    Predicted band size : 25 kDa
  • Anti-SOD2/MnSOD antibody (ab118340) at 0.1 µg/ml (incubation time: 1 hour) + Mouse Spinal Cord lysate in RIPA buffer at 35 µg
    Developed using the ECL technique

    Predicted band size : 25 kDa

プロトコール

Anti-SOD2/MnSOD antibody (ab118340) 使用論文

This product has been referenced in:
  • Huang Y  et al. Biological effects of pyrroloquinoline quinone on liver damage in Bmi-1 knockout mice. Exp Ther Med 10:451-458 (2015). Read more (PubMed: 26622336) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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