The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Perform heat-mediated antigen retrieval with 10mM citrate buffer for 10 minutes, followed by cooling at room temperature for 20 minutes.
機能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. May be a substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
パスウェイProtein modification; protein ubiquitination.