SOCS5 overexpression 293T lysate (whole cell) (ab94296)

製品の概要

  • 製品名SOCS5 overexpression 293T lysate (whole cell)
  • 製品の詳細
    SOCS5 overexpression lysate
  • 特記事項ab94296 is a 293T cell transfected lysate in which Human SOCS5 has been transiently over-expressed using a pCMV-SOCS5 plasmid. The lysate is provided in 1 x Sample Buffer. Note: For more details about how the transfected lysate was prepared view preparation notes
  • アプリケーション適用あり: WBmore details

法規制情報

製品の特性

  • 背景The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. During Th1 differentiation a reduction in the association of Jak1 with the IL4 Receptor correlated with the appearance of SOCS5. SOCS5 protein was preferentially expressed in committed Th1 cells and interacted with the cytoplasmic region of the IL4 Receptor alpha chain irrespective of receptor tyrosine phosphorylation. This unconventional interaction of SOCS5 protein with IL4 Receptor resulted in the inhibition of IL4-mediated signal transducer and activator of transcription-6 activation. T cells from transgenic mice constitutively expressing SOCS5 exhibited a significant reduction of IL4-mediated Th2 development. Therefore, the induced SOCS5 protein in Th1 differentiation environment may play an important role by regulating Th1 and Th2 balance.

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab94296 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

SOCS5 overexpression 293T lysate (whole cell) 画像

  • ab94296 at 15µg/lane on an SDS-PAGE gel.
  • All lanes : Anti-SOCS5 antibody (ab88096) at 1/500 dilution

    Lane 1 : SOCS5 overexpression 293T lysate (whole cell) (ab94296)
    Lane 2 : 293T non-transfected lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

SOCS5 overexpression 293T lysate (whole cell) (ab94296) 使用論文

ab94296 has not yet been referenced specifically in any publications.

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