製品の概要

  • 製品名Anti-SOCS5 antibody
    SOCS5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOCS5
  • 特異性This antibody reacts predominantly with SOCS 5. It was evaluated for specificity with a dot blot assay using synthetically prepared SOCS peptides: it only recognizes SOCS 5, no other SOCS.
  • アプリケーション適用あり: IHC-P, WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide corresponding to C-terminus of human/ mouse SOCS 5.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPBS with 0.02% sodium azide
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab3695 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 10 - 20 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 64 kDa (predicted molecular weight: 64 kDa).
IP Use 3-5µg for 107 cells.
ELISA Use a concentration of 0.1 - 1 µg/ml.

ターゲット情報

  • 関連性The eight members of the recently identified Suppressor of Cytokines Signaling (SOCS) family are SOCS1, SOCS2, SOCS3, SOCS4, SOCS5, SOCS6, SOCS7, and CIS. Structurally the SOCS proteins are composed of an N- terminal region of variable length and amino acid composition, a central SH2 domain, and a C-terminal motif called the SOCS box. The SOCS proteins appear to form part of a classical negative feedback loop that regulates cytokine signal transduction. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. During Th1 differentiation a reduction in the association of Jak1 with the IL4 Receptor correlated with the appearance of SOCS5. SOCS5 protein was preferentially expressed in committed Th1 cells and interacted with the cytoplasmic region of the IL4 Receptor alpha chain irrespective of receptor tyrosine phosphorylation. This unconventional interaction of SOCS5 protein with IL4 Receptor resulted in the inhibition of IL4-mediated signal transducer and activator of transcription-6 activation. T cells from transgenic mice constitutively expressing SOCS5 exhibited a significant reduction of IL4-mediated Th2 development. Therefore, the induced SOCS5 protein in Th1 differentiation environment may play an important role by regulating Th1 and Th2 balance.
  • 参照データベース
  • 別名
    • CIS-6 antibody
    • CIS6 antibody
    • CISH5 antibody
    • CISH6 antibody
    • Cytokine inducible SH2 containing protein 5 antibody
    • Cytokine inducible SH2 protein 6 antibody
    • KIAA0671 antibody
    • SOCS-5 antibody
    • Suppressor of cytokine signaling 5 antibody
    see all

Anti-SOCS5 antibody 画像

  • ab3695 staining formalin-fixed, paraffin embeded tissue section of cardiac myocytes within human heart. The primary antibody was diluted 10 μg/ml and it was detected by ABC kit. Nuclears are counterstained with hematoxylin purple.

Anti-SOCS5 antibody (ab3695) 使用論文

ab3695 has not yet been referenced specifically in any publications.

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