製品の概要

  • 製品名
  • 製品の詳細
    Goat polyclonal to SOCS3
  • アプリケーション
    適用あり: WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat
  • 免疫原

    Synthetic peptide: PIREFLDQYDAPL, corresponding to C terminal amino acids 213-225 of Human SOCS3 .

  • ポジティブ・コントロール
    • MOLT-4 lysate
  • 特記事項
    Principal Names - SOCS3; CIS3; Cish3; SSI-3; SOCS-3; suppressor of cytokine signaling 3; STAT induced STAT inhibitor 3; cytokine-induced SH2 protein 3 Official Gene Symbol - SOCS3 GenBank Accession Number – NP_003946 LocusLink ID - 9021 (human); 12702 (mouse); 89829 (rat) Gene Ontology terms - protein kinase inhibitor; JAK-STAT cascade; anti-apoptosis Size - 225 aa

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab723 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    Western Blot - Approx 24kDa band seen in MOLT-4 lysate. Recommended for use at 4.0-6.0µg/ml. Block membrane in 2.5% skimmed milk in TBST (TBS + 0.05% Tween). Incubate primary antibody for 1 hour at room temperature with agitation.
  • ターゲット情報

    • 機能
      SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
    • 組織特異性
      Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
    • パスウェイ
      Protein modification; protein ubiquitination.
    • 関連疾患
      Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
    • 配列類似性
      Contains 1 SH2 domain.
      Contains 1 SOCS box domain.
    • ドメイン
      The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
      The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
    • 翻訳後修飾
      Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
    • Information by UniProt
    • 参照データベース
    • 別名
      • ATOD4 antibody
      • CIS 3 antibody
      • CIS-3 antibody
      • CIS3 antibody
      • Cish3 antibody
      • Cytokine induced SH2 protein 3 antibody
      • Cytokine-inducible SH2 protein 3 antibody
      • E2a Pbx1 target gene in fibroblasts 10 antibody
      • EF 10 antibody
      • MGC71791 antibody
      • SOCS 3 antibody
      • SOCS-3 antibody
      • Socs3 antibody
      • SOCS3_HUMAN antibody
      • SSI 3 antibody
      • SSI-3 antibody
      • SSI3 antibody
      • STAT induced STAT inhibitor 3 antibody
      • STAT-induced STAT inhibitor 3 antibody
      • Suppressor of cytokine signaling 3 antibody
      see all

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    参考文献

    This product has been referenced in:
    • Minter MR  et al. Deletion of the type-1 interferon receptor in APPSWE/PS1?E9 mice preserves cognitive function and alters glial phenotype. Acta Neuropathol Commun 4:72 (2016). WB . Read more (PubMed: 27400725) »

    See 1 Publication for this product

    レビューと Q&A

    Thank you for your email. Ab723 is 325 Euros and is in stock in our office in Great Britain so upon ordering, you should receive it within a couple of days. You can place your order online, or via fax, email or phone. Below is the appropriate contact d...

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    Thank you for your enquiry. All the information we have on species cross reactivity is specified on the datasheet, these are updated as soon as any new information is brought to our attention. As far as we are aware, cross reactivity with rat has no...

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    Thank you for the details that you have provided. We have had no other complaints concerning this antibody. The antibody's originator has provided me with the following protocol that they used when testing the antibody which should be helpful for you: ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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