製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SOCS3
  • アプリケーション
    適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (

    QLPGPIREFLDQYDAPL

    ), corresponding to amino acids 209-225 of the human/ rat/ mouse SOCS 3.

  • ポジティブ・コントロール
    • Cardiac myocytes and A431 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 20 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IP Use 3-5µg for 107 cells.

ターゲット情報

  • 機能
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
  • 組織特異性
    Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 関連疾患
    Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
  • 配列類似性
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • ドメイン
    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATOD4 antibody
    • CIS 3 antibody
    • CIS-3 antibody
    • CIS3 antibody
    • Cish3 antibody
    • Cytokine induced SH2 protein 3 antibody
    • Cytokine-inducible SH2 protein 3 antibody
    • E2a Pbx1 target gene in fibroblasts 10 antibody
    • EF 10 antibody
    • MGC71791 antibody
    • SOCS 3 antibody
    • SOCS-3 antibody
    • Socs3 antibody
    • SOCS3_HUMAN antibody
    • SSI 3 antibody
    • SSI-3 antibody
    • SSI3 antibody
    • STAT induced STAT inhibitor 3 antibody
    • STAT-induced STAT inhibitor 3 antibody
    • Suppressor of cytokine signaling 3 antibody
    see all

画像

  • Localization of ab3693 as the precipitated red signal, with a hematoxylin purple nuclear counterstain in cardiac myocytes.


  • Predicted band size : 27 kDa
    A431 whole cell lysate was probed with rabbit anti-SOCS-3 ab3693 at 1:200 dilution. A431 whole cell lysate was probed with rabbit anti-SOCS-3 (ab3693) at 1:200 dilution.
  • ab3693 (2µg/ml) staining SOCS3 in human jejunum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cells within the lamina propria.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

参考文献

This product has been referenced in:
  • Roncero AM  et al. Contribution of JAK2 mutations to T-cell lymphoblastic lymphoma development. Leukemia 30:94-103 (2016). WB ; Human . Read more (PubMed: 26216197) »
  • Akram KM  et al. Alveolar epithelial cells in idiopathic pulmonary fibrosis display upregulation of TRAIL, DR4 and DR5 expression with simultaneous preferential over-expression of pro-apoptotic marker p53. Int J Clin Exp Pathol 7:552-64 (2014). IHC-P ; Human . Read more (PubMed: 24551275) »

See all 14 Publications for this product

レビューと Q&A

Filter by Application

Filter by Species

Filter by Ratings

Abreviews
Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (primary human macrophage)
Specification
primary human macrophage
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 19 2013

Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (A549 lung carcinoma cells)
Loading amount
150000 cells
Specification
A549 lung carcinoma cells
Gel Running Conditions
Reduced Denaturing (15 % acryl amide)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Michel Crameri

Verified customer

投稿 Sep 25 2012

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Macrophages in liver , kidney & tonsil)
Specification
Macrophages in liver , kidney & tonsil
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated
Permeabilization
No
Username

Abcam user community

Verified customer

投稿 Aug 10 2012

Abreviews
Application
Western blot
Sample
Human Cell lysate - nuclear (Primary HPMC)
Loading amount
5 µg
Specification
Primary HPMC
Gel Running Conditions
Reduced Denaturing (7.5 %)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Robert Jenkins

Verified customer

投稿 Jun 01 2009

Abreviews
Application
Western blot
Sample
Rat Tissue lysate - whole (skin)
Loading amount
15 µg
Specification
skin
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5%
Username

Abcam user community

Verified customer

投稿 Feb 05 2007

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Mouse Cell lysate - whole cell (RAW 264.7 monocytes)
Specification
RAW 264.7 monocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Dr. Minh Nguyen

Verified customer

投稿 Oct 26 2005

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

登録