製品の概要

  • 製品名Anti-SOCS3 antibody
    SOCS3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOCS3
  • アプリケーション適用あり: IHC-P, WB, IPmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat
  • 免疫原

    Synthetic peptide (

    QLPGPIREFLDQYDAPL

    ), corresponding to amino acids 209-225 of the human/ rat/ mouse SOCS 3.

  • ポジティブ・コントロール
    • Cardiac myocytes and A431 whole cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab3693 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 20 µg/ml.
WB Use at an assay dependent concentration. Detects a band of approximately 27 kDa (predicted molecular weight: 27 kDa).
IP Use 3-5µg for 107 cells.

ターゲット情報

  • 機能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
  • 組織特異性Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
  • パスウェイProtein modification; protein ubiquitination.
  • 関連疾患Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
  • 配列類似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • ドメインThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • 翻訳後修飾Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATOD4 antibody
    • CIS 3 antibody
    • CIS-3 antibody
    • CIS3 antibody
    • Cish3 antibody
    • Cytokine induced SH2 protein 3 antibody
    • Cytokine-inducible SH2 protein 3 antibody
    • E2a Pbx1 target gene in fibroblasts 10 antibody
    • EF 10 antibody
    • MGC71791 antibody
    • SOCS 3 antibody
    • SOCS-3 antibody
    • Socs3 antibody
    • SOCS3_HUMAN antibody
    • SSI 3 antibody
    • SSI-3 antibody
    • SSI3 antibody
    • STAT induced STAT inhibitor 3 antibody
    • STAT-induced STAT inhibitor 3 antibody
    • Suppressor of cytokine signaling 3 antibody
    see all

Anti-SOCS3 antibody 画像

  • Localization of ab3693 as the precipitated red signal, with a hematoxylin purple nuclear counterstain in cardiac myocytes.


  • Predicted band size : 27 kDa
    A431 whole cell lysate was probed with rabbit anti-SOCS-3 ab3693 at 1:200 dilution. A431 whole cell lysate was probed with rabbit anti-SOCS-3 (ab3693) at 1:200 dilution.
  • ab3693 (2µg/ml) staining SOCS3 in human jejunum using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cells within the lamina propria.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.

Anti-SOCS3 antibody (ab3693) 使用論文

This product has been referenced in:
  • Akram KM  et al. Alveolar epithelial cells in idiopathic pulmonary fibrosis display upregulation of TRAIL, DR4 and DR5 expression with simultaneous preferential over-expression of pro-apoptotic marker p53. Int J Clin Exp Pathol 7:552-64 (2014). IHC-P ; Human . Read more (PubMed: 24551275) »
  • Bonetto A  et al. STAT3 Activation in Skeletal Muscle Links Muscle Wasting and the Acute Phase Response in Cancer Cachexia. PLoS One 6:e22538 (2011). WB ; Mouse . Read more (PubMed: 21799891) »

See all 13 Publications for this product

Product Wall

Abreviews
Application Western blot
Loading amount 1e+006 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (primary human macrophage)
Specification primary human macrophage
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 19 2013


In general it would be ideal to have a positive control as perhaps SOCS3 is not expressed in A549 cells. We have another antibody go SOCS3, ab16030, where the antibody shows detection of a band in a number of common tumor cell types, Hela, Jurkat,...

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You mentioned using another antibody to SOCS3 which did not produce the thick band at 37kDa. Did that antibody produce a band just at 27kDa?

Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (A549 lung carcinoma cells)
Loading amount 150000 cells
Specification A549 lung carcinoma cells
Gel Running Conditions Reduced Denaturing (15 % acryl amide)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Mr. Michel Crameri

Verified customer

投稿 Sep 25 2012

Thanks for your email.


It was a good idea to use the "EasyBlocker" reagent. It makes it quite clear that there is no SOCS3 being pulled down (IP'd) using ab3693, although the antibody does function well for western blotting al...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (Macrophages in liver , kidney & tonsil)
Specification Macrophages in liver , kidney & tonsil
Fixative Paraformaldehyde
Antigen retrieval step Heat mediated
Permeabilization No
Username

Abcam user community

Verified customer

投稿 Aug 10 2012

Thanks so much for providing the additional details. I just have a few more follow up questions:

3. Based on the included WB image, it seems SOCS3 is detected from the input via WB but not the IP/WB. Do you agree? NO
==>If you could, ...

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Thank you for your enquiry and for including a western blot image. I just have a few additional questions:

1. This seems to be an IP/WB experiment. Is this the case?

2. Is ab3693 used for both IP and WB? If not, which antibodies are...

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Thank you for your reply.

I was wandering if the fellow who ordered the product would be able to provide some of the original order information? As I mentioned in my previous emails, I am willing to extend the 6 month Abpromise for a few mont...

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Thank you for your reply.

I understand your situation, but I do need to have some indication about when ab3693 was actually ordered. A date, name or PO number would work.

As I mentioned previously, I am willing to extend the Abpromi...

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