製品の概要

  • 製品名Anti-SOCS3 antibody
    SOCS3 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOCS3
  • アプリケーション適用あり: ICC/IF, Flow Cyt, WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human SOCS3.

    (Peptide available as ab16199.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: Hela, Jurkat, A431 and MOLT4.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt 1/300. ab171870-Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24.7 kDa).Can be blocked with Human SOCS3 peptide (ab16199).
IHC-Fr Use at an assay dependent concentration. PubMed: 19855398
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

ターゲット情報

  • 機能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
  • 組織特異性Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
  • パスウェイProtein modification; protein ubiquitination.
  • 関連疾患Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
  • 配列類似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • ドメインThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • 翻訳後修飾Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATOD4 antibody
    • CIS 3 antibody
    • CIS-3 antibody
    • CIS3 antibody
    • Cish3 antibody
    • Cytokine induced SH2 protein 3 antibody
    • Cytokine-inducible SH2 protein 3 antibody
    • E2a Pbx1 target gene in fibroblasts 10 antibody
    • EF 10 antibody
    • MGC71791 antibody
    • SOCS 3 antibody
    • SOCS-3 antibody
    • Socs3 antibody
    • SOCS3_HUMAN antibody
    • SSI 3 antibody
    • SSI-3 antibody
    • SSI3 antibody
    • STAT induced STAT inhibitor 3 antibody
    • STAT-induced STAT inhibitor 3 antibody
    • Suppressor of cytokine signaling 3 antibody
    see all

Anti-SOCS3 antibody 画像

  • All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 4 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
    Lane 5 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
    Lane 6 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
    Lane 7 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate with Human SOCS3 peptide (ab16199) at 1 µg/ml
    Lane 8 : MOLT4 (Human) Whole Cell Lysate (ab52252) with Human SOCS3 peptide (ab16199) at 1 µg/ml

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Performed under reducing conditions.

    Observed band size : 30 kDa (why is the actual band size different from the predicted?)
  • ICC/IF image of ab16030 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab16030, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Image courtesy of Human Protein Atlas

    ab16030 staining SOCS3 in human bone marrow showing strong staining within the bone marrow. Paraffin embedded human bone marrow was incubated with ab16030 (1/100) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH6.

    ab16030 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • ab16030 staining SOCS3 in Human pancreatic tumour tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained by DAPI.

    See Abreview

  • All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml

    Lane 1 : Untreated Human peripheral blood mononuclear cell lysate
    Lane 2 : Human peripheral blood mononuclear cell lysate (treated with aCD3/28)
    Lane 3 : Human peripheral blood mononuclear cell lysate (treated with IFNγ)
    Lane 4 : SOCS3 protein

    Lysates/proteins at 15 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24.7 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

Anti-SOCS3 antibody (ab16030) 使用論文

This product has been referenced in:
  • Kim MH  et al. Suppressor of cytokine signaling (SOCS) genes are silenced by DNA hypermethylation and histone deacetylation and regulate response to radiotherapy in cervical cancer cells. PLoS One 10:e0123133 (2015). IHC ; Human . Read more (PubMed: 25849377) »
  • Trilling M  et al. "Activated" STAT proteins: a paradoxical consequence of inhibited JAK-STAT signaling in cytomegalovirus-infected cells. J Immunol 192:447-58 (2014). Mouse . Read more (PubMed: 24319264) »

See all 24 Publications for this product

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Abcam has not validated the combination of species/application used in this Abreview.
Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Cat Tissue sections (liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization No
Specification liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
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投稿 May 20 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Human Tissue sections (liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization No
Specification liver
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 03 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Horse Tissue sections (trachea lymphoma)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization No
Specification trachea lymphoma
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 03 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample Dog Tissue sections (liver)
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
Permeabilization No
Specification liver
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 29 2016

Application Immunohistochemistry (Frozen sections)
Sample Horse Tissue sections (Lymph node)
Permeabilization No
Specification Lymph node
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Feb 06 2016

Application Immunohistochemistry (Frozen sections)
Sample Dog Tissue sections (Lymph node)
Permeabilization No
Specification Lymph node
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative Acetone
Username

Abcam user community

Verified customer

投稿 Feb 01 2016

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer, pH6.0
Sample Rat Tissue sections (sciatic nerve (injured with lymphatic cells penetr)
Specification sciatic nerve (injured with lymphatic cells penetr
Permeabilization No
Fixative Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 28 2014

Application Western blot
Loading amount 20 µg
Gel Running Conditions Reduced Denaturing
Sample Mouse Cell lysate - whole cell (LIVER)
Specification LIVER
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jul 29 2014

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Sample Mouse Tissue sections (liver)
Specification liver
Permeabilization No
Fixative Formaldehyde
Username

Xiaoyu Wang

Verified customer

投稿 Jun 04 2014

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