製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SOCS3
  • アプリケーション
    適用あり: ICC/IF, Flow Cyt, WB, IHC-Fr, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Chicken, Cow, Dog
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human SOCS3.

    (Peptide available as ab16199.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following Human Whole Cell Lysates: Hela, Jurkat, A431 and MOLT4.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab16030 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt 1/300.

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB Use a concentration of 1 µg/ml. Detects a band of approximately 30 kDa (predicted molecular weight: 24.7 kDa).Can be blocked with Human SOCS3 peptide (ab16199).
IHC-Fr Use at an assay dependent concentration. PubMed: 19855398
IHC-P 1/100. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.

ターゲット情報

  • 機能
    SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
  • 組織特異性
    Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 関連疾患
    Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
  • 配列類似性
    Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • ドメイン
    The ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • 翻訳後修飾
    Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATOD4 antibody
    • CIS 3 antibody
    • CIS-3 antibody
    • CIS3 antibody
    • Cish3 antibody
    • Cytokine induced SH2 protein 3 antibody
    • Cytokine-inducible SH2 protein 3 antibody
    • E2a Pbx1 target gene in fibroblasts 10 antibody
    • EF 10 antibody
    • MGC71791 antibody
    • SOCS 3 antibody
    • SOCS-3 antibody
    • Socs3 antibody
    • SOCS3_HUMAN antibody
    • SSI 3 antibody
    • SSI-3 antibody
    • SSI3 antibody
    • STAT induced STAT inhibitor 3 antibody
    • STAT-induced STAT inhibitor 3 antibody
    • Suppressor of cytokine signaling 3 antibody
    see all

画像

  • ICC/IF image of ab16030 stained human HeLa cells. The cells were PFA fixed (10 min) and incubated with the antibody (ab16030, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

  • Image courtesy of Human Protein Atlas

    ab16030 staining SOCS3 in human bone marrow showing strong staining within the bone marrow. Paraffin embedded human bone marrow was incubated with ab16030 (1/100) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH6.

    ab16030 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org

  • ab16030 staining SOCS3 in Human pancreatic tumour tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with paraformaldehyde and blocked with 5% serum for 1 hour at room temperature. Samples were incubated with primary antibody (1/300 in PBS) for 8 hours at 4°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/1000) was used as the secondary antibody. Nuclei were stained by DAPI.

    See Abreview

  • All lanes : Anti-SOCS3 antibody (ab16030) at 1 µg/ml

    Lane 1 : Untreated Human peripheral blood mononuclear cell lysate
    Lane 2 : Human peripheral blood mononuclear cell lysate (treated with aCD3/28)
    Lane 3 : Human peripheral blood mononuclear cell lysate (treated with IFNγ)
    Lane 4 : SOCS3 protein

    Lysates/proteins at 15 µg per lane.

    Secondary
    HRP-conjugated goat anti-rabbit polyclonal IgG at 1/2000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 24.7 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)


    Exposure time : 15 minutes

    This image is courtesy of an anonymous Abreview

    See Abreview

参考文献

This product has been referenced in:
  • Hwang M  et al. Epac2a-null mice exhibit obesity-prone nature more susceptible to leptin resistance. Int J Obes (Lond) 41:279-288 (2017). WB ; Mouse . Read more (PubMed: 27867203) »
  • Zheng XB  et al. miR-203 inhibits augmented proliferation and metastasis of hepatocellular carcinoma residual in the promoted regenerating liver. Cancer Sci 108:338-346 (2017). Read more (PubMed: 28100026) »

See all 47 Publications for this product

レビューと Q&A

Thank you for confirming these details and for your cooperation. The details provided enable us to closely monitor the quality of our products.

I am sorry that this specific lot did not perform as stated on the datasheet and for the inconven...

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Cat Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
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Verified customer

投稿 May 20 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 03 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Horse Tissue sections (trachea lymphoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate, pH6.0
Permeabilization
No
Specification
trachea lymphoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Mar 03 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (liver)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: tris-EDTA, pH9.0
Permeabilization
No
Specification
liver
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Feb 29 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Horse Tissue sections (Lymph node)
Permeabilization
No
Specification
Lymph node
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

投稿 Feb 06 2016

Application
Immunohistochemistry (Frozen sections)
Sample
Dog Tissue sections (Lymph node)
Permeabilization
No
Specification
Lymph node
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Acetone
Username

Abcam user community

Verified customer

投稿 Feb 01 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer, pH6.0
Sample
Rat Tissue sections (sciatic nerve (injured with lymphatic cells penetr)
Specification
sciatic nerve (injured with lymphatic cells penetr
Permeabilization
No
Fixative
Formaldehyde
Username

Abcam user community

Verified customer

投稿 Aug 28 2014

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Mouse Cell lysate - whole cell (LIVER)
Specification
LIVER
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Username

Abcam user community

Verified customer

投稿 Jul 29 2014

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2.5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate Buffer pH 6.0
Sample
Mouse Tissue sections (liver)
Specification
liver
Permeabilization
No
Fixative
Formaldehyde
Username

Xiaoyu Wang

Verified customer

投稿 Jun 04 2014

1-10 of 48 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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