製品の概要

  • 製品名Anti-SOCS3 antibody [1B2]
    SOCS3 一次抗体 製品一覧
  • 製品の詳細
    Mouse monoclonal [1B2] to SOCS3
  • アプリケーション適用あり: Flow Cyt, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Chicken, Cow, Dog, Pig
  • 免疫原

    Recombinant fusion protein: VTHSKFPAAG MSRPLDTSLR LKTFSSKSEY QLVVNAVRKL QES, corresponding to amino acids 2-44 of Mouse SOCS3

  • エピトープN terminal
  • ポジティブ・コントロール
    • Lysate from HeLa cells treated with IFN alpha. Lysate from EGF-treated A431 cells. This antibody gave a positive result in IHC in the following FFPE tissue: Human Lung.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab78341 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use 1µg for 106 cells. ab170190-Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
WB 1/1000. Detects a band of approximately 25 kDa (predicted molecular weight: 27 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

ターゲット情報

  • 機能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST.
  • 組織特異性Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
  • パスウェイProtein modification; protein ubiquitination.
  • 関連疾患Note=There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
  • 配列類似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • ドメインThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • 翻訳後修飾Phosphorylated on tyrosine residues after stimulation by the cytokines, IL-2, EPO or IGF1.
  • Information by UniProt
  • 参照データベース
  • 別名
    • ATOD4 antibody
    • CIS 3 antibody
    • CIS-3 antibody
    • CIS3 antibody
    • Cish3 antibody
    • Cytokine induced SH2 protein 3 antibody
    • Cytokine-inducible SH2 protein 3 antibody
    • E2a Pbx1 target gene in fibroblasts 10 antibody
    • EF 10 antibody
    • MGC71791 antibody
    • SOCS 3 antibody
    • SOCS-3 antibody
    • Socs3 antibody
    • SOCS3_HUMAN antibody
    • SSI 3 antibody
    • SSI-3 antibody
    • SSI3 antibody
    • STAT induced STAT inhibitor 3 antibody
    • STAT-induced STAT inhibitor 3 antibody
    • Suppressor of cytokine signaling 3 antibody
    see all

Anti-SOCS3 antibody [1B2] 画像

  • IHC image of SOCS3 staining in Human Lung formalin fixed paraffin embedded tissue section, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval (EDTA based pH 9.0 solution, epitope retrieval solution 2) for 20 mins. The section was then incubated with ab78341, 1 µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-SOCS3 antibody [1B2] (ab78341) at 1/1000 dilution + IFN alpha-treated HeLa cell lysate

    Secondary
    Goat anti-mouse HRP conjugate at 1/5000 dilution

    Predicted band size : 27 kDa
    Observed band size : 25 kDa (why is the actual band size different from the predicted?)
  • Overlay histogram showing Jurkat cells stained with ab78341 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab78341, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Unlabelled sample (blue line). Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in Jurkat cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

Anti-SOCS3 antibody [1B2] (ab78341) 使用論文

ab78341 has not yet been referenced specifically in any publications.

Product Wall

There are currently no Abreviews or Questions for ab78341.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"