製品の概要

  • 製品名Anti-SOCS2 antibody
    SOCS2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOCS2
  • 特異性By Western blot, this antibody detects a band of approximately 23 kDa in lysate of GH treated HEK 293 cells transfected with GHR, which corresponds to the predicted molecular weight of SOCS 2.
  • アプリケーション適用あり: WB, IP, ELISAmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide (

    PTRLKDYLEEYKFQV

    ) corresponding to amino acids 184-198 of human, rat and mouse SOCS 2.

  • ポジティブ・コントロール
    • lysate of GH treated HEK 293 cells transfected with GHR, E17 mouse cortical neuron lysate (see Scott et al)
  • 特記事項


    Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 2, not like SOCS 1 and SOCS 3, regulates postnatal growth, likely through its ability to influence the GH / IGF 1axis, although they might have overlapping actions.

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファーPBS with 0.02% sodium azide
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 一次抗体 備考Accumulating evidence demonstrates that cytokine receptor signaling is negatively regulated by a family of Src homology 2 domain-containing adaptor molecules Termed SOCS (Suppressor of Cytokine Signaling). To date, there are eight members of SOCS family have been recognized, they are SOCS 1, 2, 3, 4, 5, 6, 7 and CIS. Structurally the SOCS proteins are composed of an N-terminal region of variable length and amino acid composition, a central SH2 domain, and a previously unrecognized C-terminal motif that we have called the SOCS box. The SOCS proteins appear to form part of a classical negative feed back loop that regulates cytokine signal transduction via a STAT induced transcriptional mechanism. Transcription of each of the SOCS genes occurs rapidly in vitro and in vivo in response to cytokines, and once produced, the various members of the SOCS family appear to inhibit signaling in different ways. SOCS 2, not like SOCS 1 and SOCS 3, regulates postnatal growth, likely through its ability to influence the GH / IGF 1axis, although they might have overlapping actions.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab3692 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 23 kDa (predicted molecular weight: 23 kDa).
IP Use 3-5µg for 107 cells.
ELISA Use a concentration of 0.1 - 1 µg/ml.

ターゲット情報

  • 機能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS2 appears to be a negative regulator in the growth hormone/IGF1 signaling pathway. Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins.
  • 組織特異性High expression in heart, placenta, lung, kidney and prostate.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • ドメインThe SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CIS 2 antibody
    • CIS-2 antibody
    • CIS2 antibody
    • Cish 2 antibody
    • Cish2 antibody
    • Cytokine inducible SH2 protein 2 antibody
    • Cytokine-inducible SH2 protein 2 antibody
    • SOCS 2 antibody
    • SOCS-2 antibody
    • Socs2 antibody
    • SOCS2_HUMAN antibody
    • SSI 2 antibody
    • SSI-2 antibody
    • SSI2 antibody
    • STAT induced STAT inhibitor 2 antibody
    • STAT-induced STAT inhibitor 2 antibody
    • STATI 2 antibody
    • STATI2 antibody
    • Suppressor of cytokine signaling 2 antibody
    see all

Anti-SOCS2 antibody 画像



  • Predicted band size : 23 kDa

    Image from Gilli F et al., PLoS One. 2010 Jan 29;5(1):e8962. Fig 4.; doi:10.1371/journal.pone.0008962; PLoS ONE 5(1): e8962.

Anti-SOCS2 antibody (ab3692) 使用論文

This product has been referenced in:
  • Gurda GT  et al. Profiling CCK-mediated pancreatic growth: the dynamic genetic program and the role of STATs as potential regulators. Physiol Genomics 44:14-24 (2012). WB ; Mouse . Read more (PubMed: 22010007) »
  • Gilli F  et al. Learning from nature: pregnancy changes the expression of inflammation-related genes in patients with multiple sclerosis. PLoS One 5:e8962 (2010). WB ; Human . Read more (PubMed: 20126412) »

See all 9 Publications for this product

Product Wall

Application Western blot
Loading amount 30 µg
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (macrophage)
Specification macrophage
Treatment 0.1 ug/ml LPS 24 h
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Dec 22 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Loading amount 50 cells
Gel Running Conditions Reduced Denaturing
Sample Human Cell lysate - whole cell (primary human macrophages)
Specification primary human macrophages
Treatment MDP 100ug/ml
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 25°C
Username

Dr. sha zheng

Verified customer

投稿 Jun 18 2013

Thank you for passing on this enquiry from the customer. I hope you are well. All our antibodies that have been tested western blotting will detect reduced proteins, unless otherwise stated the datasheet. The customer will therefore need to reduce ...

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Thank you for getting back to me with your customer's further information. Please can you pass on the following comments to your customer: I am surprised that you have been unable to detect consistent hypothalamus staining using this antibody. It i...

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Thank you for your enquiry. I am sorry to hear that you have been having difficulties with this antibody. Quality is very important to Abcam. We take all of our complaints very seriously and will not hesitate to remove an antibody should we have co...

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Thank you for your enquiry. Unfortunately we do no have a recommended positive control for SOCS1 antibody (ab3691). However, the customer that left a favourable Abreview used a human cell lysate - whole cell (human malignant melanoma cells). Furthe...

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I have received feedback from the source of ab3692 stating that unfortunately they do not know if the cells were transfected with the receptor or not. We do not have the stimulation paradigm information unfortunately, I'm very sorry. From your literatu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"