製品の概要

  • 製品名Anti-SOCS1 antibody
    SOCS1 一次抗体 製品一覧
  • 製品の詳細
    Goat polyclonal to SOCS1
  • アプリケーション適用あり: IHC-P, WB, ICC/IF, Flow Cytmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow, Dog
  • 免疫原

    Synthetic peptide: VLRDYLSSFPFQI, corresponding to C terminal amino acids 199-211 of Human SOCS1 .

  • ポジティブ・コントロール
    • Mouse Spleen Lysate
  • 特記事項Principal Names - SSI-1; JAK binding protein; JAB; SOCS1; SOCS-1; CIS1; SSI1 Official Gene Symbol - SOCS1 GenBank Accession Number – NP_003736 LocusLink ID - 8651

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab9870 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 2 - 3 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
WB Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 30-35 kDa.Can be blocked with Human SOCS1 peptide (ab23167).
ICC/IF Use at an assay dependent concentration. PubMed: 21628332
Flow Cyt Use at an assay dependent concentration. PubMed: 23298838ab37373-Goat polyclonal IgG, is suitable for use as an isotype control with this antibody.

ターゲット情報

  • 機能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
  • 組織特異性Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • ドメインThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CISH 1 antibody
    • CISH1 antibody
    • Cytokine inducible SH2 protein 1 antibody
    • JAB antibody
    • JAK binding protein antibody
    • JAK-binding protein antibody
    • Janus kinase binding protein antibody
    • SOCS 1 antibody
    • SOCS-1 antibody
    • Socs1 antibody
    • SOCS1_HUMAN antibody
    • SSI 1 antibody
    • SSI-1 antibody
    • SSI1 antibody
    • STAT induced STAT inhibitor 1 antibody
    • STAT-induced STAT inhibitor 1 antibody
    • Suppressor of cytokine signaling 1 antibody
    • Supressor of cytokine signalling 1 antibody
    • TEC interacting protein 3 antibody
    • Tec-interacting protein 3 antibody
    • TIP 3 antibody
    • TIP-3 antibody
    • TIP3 antibody
    see all

Anti-SOCS1 antibody 画像

  • ab9870 (2.5µg/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
  • This picture was kindly supplied as part of the review submitted by Minh Nguyen.
  • HEK293 overexpressing SOCS1 and probed with ab9870 (mock transfection in lane B).
  • Anti-SOCS1 antibody (ab9870) at 2 µg/ml + Mouse Spleen Lysate in RIPA Buffer at 35 µg

Anti-SOCS1 antibody (ab9870) 使用論文

This product has been referenced in:
  • Arnold CE  et al. A critical role for suppressor of cytokine signalling 3 in promoting M1 macrophage activation and function in vitro and in vivo. Immunology 141:96-110 (2014). IHC . Read more (PubMed: 24088176) »
  • Kim SJ  et al. Regulation of dendritic cell activation by microRNA let-7c and BLIMP1. J Clin Invest N/A:N/A (2013). Flow Cyt ; Mouse . Read more (PubMed: 23298838) »

See all 5 Publications for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (monocyte-derived macrophage)
Loading amount 30 µg
Specification monocyte-derived macrophage
Gel Running Conditions Reduced Denaturing (10)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

Abcam user community

Verified customer

投稿 Jan 17 2013

Thank you for contacting us.

To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.

http://www.abcam.com/index.html?pageconfig=resource&rid=1...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Rat Cell lysate - whole cell (rat Bone marrow derived macrophages)
Loading amount 15 µg
Specification rat Bone marrow derived macrophages
Treatment stimulants of macrophage activation for 4 hours
Gel Running Conditions Reduced Denaturing (12% gel)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Apr 04 2008

Thank you for your enquiry. The differences between goat polyclonal to SOCS1 (ab9870) and rabbit polyclonal to SOCS1 (ab3691) is that ab9870 reacts with mouse and is predicted to react with human due to sequence homology. So far it has only been sh...

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Thank you for your patience. As ab5923 was originated outside Abcam I had to contact the originator regarding your question and have just heard back. There was a mistake on the datasheet for ab5923 - it is a CIS1 antibody, not a SOCS1 antibody. I ap...

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I'm sorry to hear that you are having problems with these two antibodies. I am enquiring with the originators of these two antibodies for suggestions that will help you out. In the meantime, below is a general protocol for ab9870 that will be helpful f...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"