製品の概要

  • 製品名Anti-SOCS1 antibody
    SOCS1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SOCS1
  • アプリケーション適用あり: WB, ICC/IF, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic 15 amino acid peptide from near the carboxy terminus of human SOCS1.

  • ポジティブ・コントロール
    • Human spleen cell lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab62584 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 24 kDa).
ICC/IF Use a concentration of 1 µg/ml.
IHC-P Use a concentration of 4 µg/ml.

ターゲット情報

  • 機能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
  • 組織特異性Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 SH2 domain.
    Contains 1 SOCS box domain.
  • ドメインThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
    The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box.
  • Information by UniProt
  • 参照データベース
  • 別名
    • CISH 1 antibody
    • CISH1 antibody
    • Cytokine inducible SH2 protein 1 antibody
    • JAB antibody
    • JAK binding protein antibody
    • JAK-binding protein antibody
    • Janus kinase binding protein antibody
    • SOCS 1 antibody
    • SOCS-1 antibody
    • Socs1 antibody
    • SOCS1_HUMAN antibody
    • SSI 1 antibody
    • SSI-1 antibody
    • SSI1 antibody
    • STAT induced STAT inhibitor 1 antibody
    • STAT-induced STAT inhibitor 1 antibody
    • Suppressor of cytokine signaling 1 antibody
    • Supressor of cytokine signalling 1 antibody
    • TEC interacting protein 3 antibody
    • Tec-interacting protein 3 antibody
    • TIP 3 antibody
    • TIP-3 antibody
    • TIP3 antibody
    see all

Anti-SOCS1 antibody 画像

  • Lane 1 : Anti-SOCS1 antibody (ab62584) at 1 µg/ml
    Lane 2 : Anti-SOCS1 antibody (ab62584) at 2 µg/ml
    Lane 3 : Anti-SOCS1 antibody (ab62584) at 4 µg/ml

    Lane 1 : Human spleen cell lysate
    Lane 2 : Human spleen cell lysate
    Lane 3 : Human spleen cell lysate

    Lysates/proteins at 15 µg per lane.


    Predicted band size : 24 kDa
    Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  • ab62584 (4µg/ml) staining SOCS1 in human lung using an automated system (DAKO Autostainer Plus). Using this protocol there is staining of the cytoplasmic and nuclei of macrophages and some pneumocytes. There was also cytoplasmic and nuclei staining of epithelium cells of the bronchioles.
    Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers EDTA pH 9.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
  • ICC/IF image of ab62584 stained HeLa cells. The cells were 4% formaldehyde (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab62584, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

Anti-SOCS1 antibody (ab62584) 使用論文

This product has been referenced in:
  • Kim MH  et al. Suppressor of cytokine signaling (SOCS) genes are silenced by DNA hypermethylation and histone deacetylation and regulate response to radiotherapy in cervical cancer cells. PLoS One 10:e0123133 (2015). IHC ; Human . Read more (PubMed: 25849377) »
  • Schauer IG  et al. Interleukin-1ß promotes ovarian tumorigenesis through a p53/NF-?B-mediated inflammatory response in stromal fibroblasts. Neoplasia 15:409-20 (2013). WB ; Human . Read more (PubMed: 23555186) »

See all 3 Publications for this product

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To our knowledge, customised protocols are not required for this product, any general Flow Cytometry protocol could be used with this antibody.

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