製品の概要
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製品名Anti-SOCS1 antibody
SOCS1 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to SOCS1
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由来種Rabbit
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特異性By Western blot, this antibody detects a band of approximately 24 kDa in GH treated HEK 293 cell lysate, which corresponds to the predicted molecular weight of SOCS 1. However, in human spleen cell lysate a band at 37kDa is observed.
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アプリケーション適用あり: Flow Cyt, WB, ELISAmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide:
VLRDYLSSFPFQI
, corresponding to amino acids 199-211 of human SOCS 1 or 200-212 of mouse SOCS 1. -
ポジティブ･コントロール
- human spleen lysate
製品の特性
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製品の状態Liquid
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保存方法Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPreservative: 0.02% Sodium azide
Constituents: 0.1% BSA, PBS -
Concentration information loading... -
精製度Immunogen affinity purified
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
アプリケーション
Our Abpromise guarantee covers the use of ab3691 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
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| Flow Cyt | Use at an assay dependent concentration. PubMed: 19435411 ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.
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| WB | Use a concentration of 1 - 2 µg/ml. Detects a band of approximately 38 kDa (predicted molecular weight: 24 kDa). | |
| ELISA | Use a concentration of 0.1 - 1 µg/ml. |
ターゲット情報
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機能SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS1 is involved in negative regulation of cytokines that signal through the JAK/STAT3 pathway. Through binding to JAKs, inhibits their kinase activity. In vitro, also suppresses Tec protein-tyrosine activity. Appears to be a major regulator of signaling by interleukin 6 (IL6) and leukemia inhibitory factor (LIF). Regulates interferon-gamma mediated sensory neuron survival (By similarity). Probable substrate recognition component of an ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize JAK2.
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組織特異性Expressed in all tissues with high expression in spleen, small intestine and peripheral blood leukocytes.
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パスウェイProtein modification; protein ubiquitination.
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配列類似性Contains 1 SH2 domain.
Contains 1 SOCS box domain. -
ドメインThe ESS and SH2 domains are required for JAK phosphotyrosine binding. Further interaction with the KIR domain is necessary for signal and kinase inhibition.
The SOCS box domain mediates the interaction with the Elongin BC complex, an adapter module in different E3 ubiquitin ligase complexes. The Elongin BC complex binding domain is also known as BC-box with the consensus [APST]-L-x(3)-C-x(3)-[AILV] and is part of the SOCS box. - Information by UniProt
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参照データベース
- Entrez Gene: 8651 Human
- Entrez Gene: 12703 Mouse
- Entrez Gene: 252971 Rat
- Omim: 603597 Human
- SwissProt: O15524 Human
- SwissProt: O35716 Mouse
- SwissProt: Q9QX78 Rat
- Unigene: 50640 Human
see all -
別名
- CISH 1 antibody
- CISH1 antibody
- Cytokine inducible SH2 protein 1 antibody
see all
画像
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Western blot - Anti-SOCS1 antibody (ab3691)All lanes : Anti-SOCS1 antibody (ab3691)
Lane 1 : K562 cell lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : K562 cell lysate with Immunizing peptide
Lane 4 : Mouse spleen tissue lysate with Immunizing peptide
Predicted band size: 24 kDa
参考文献
This product has been referenced in:
- Chuang Y et al. Regulation of the IL-10-driven macrophage phenotype under incoherent stimuli. Innate Immun 22:647-657 (2016). WB ; Mouse . Read more (PubMed: 27670945) »
- Webster KE et al. IL-17-producing NKT cells depend exclusively on IL-7 for homeostasis and survival. Mucosal Immunol N/A:N/A (2014). Human . Read more (PubMed: 24448098) »
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"