製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SNX3
  • アプリケーション
    適用あり: WB, ELISA, ICC/IFmore details
  • 種交差性
    交差種: Human, Recombinant fragment
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide (Human) sequence C-terminal end.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 50% Glycerol, PBS
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab56078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 19 kDa. This antibody has been tested in Western blot against the recombinant peptide used as an immunogen. We have no data on detection of endogenous protein.
ELISA Use at an assay dependent dilution.
ICC/IF Use a concentration of 1 - 5 µg/ml.

ターゲット情報

  • 機能
    Phosphoinositide-binding protein required for multivesicular body formation. Specifically binds phosphatidylinositol-3-phosphate (PtdIns(P3)). Plays a role in protein transport between cellular compartments. Promotes stability and cell surface expression of epithelial sodium channel (ENAC) subunits SCNN1A and SCNN1G (By similarity). Not involved in EGFR degradation.
  • 関連疾患
    A chromosomal aberration involving SNX3 may be a cause of microphthalmia syndromic type 8 (MCOPS8) [MIM:601349]. Translocation t(6;13)(q21;q12). Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS8 is a very rare congenital syndrome characterized by microcephaly, microphthalmia, ectrodactyly of the lower limbs and prognathism. Intellectual deficit has been reported.
  • 配列類似性
    Belongs to the sorting nexin family.
    Contains 1 PX (phox homology) domain.
  • ドメイン
    The PX domain mediates specific binding to phosphatidylinositol-3-phosphate (PtdIns(P3)).
  • 翻訳後修飾
    Ubiquitinated, leading to its proteasomal degradation. Deubiquitinated by USP10.
  • 細胞内局在
    Early endosome.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Grd19 antibody
    • MCOPS8 antibody
    • MGC151262 antibody
    • MGC151266 antibody
    • MGC17570 antibody
    • OTTHUMP00000016941 antibody
    • OTTHUMP00000046475 antibody
    • OTTHUMP00000046476 antibody
    • Protein SDP3 antibody
    • SDP3 antibody
    • SNX3 antibody
    • SNX3_HUMAN antibody
    • SNX3A antibody
    • Sorting nexin 3 antibody
    • Sorting nexin 3A antibody
    • Sorting nexin-3 antibody
    see all

画像

  • ICC/IF image of ab56078 stained Hek293 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab56078, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Morel E  et al. Phosphatidylinositol-3-phosphate regulates sorting and processing of amyloid precursor protein through the endosomal system. Nat Commun 4:2250 (2013). Read more (PubMed: 23907271) »

See 1 Publication for this product

レビューと Q&A

Application
Western blot
Loading amount
40 µg
Gel Running Conditions
Reduced Denaturing (4-20% gradient gel)
Sample
Mouse Cell lysate - whole cell (MEF, mouse Raw264 cells)
Specification
MEF, mouse Raw264 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 22°C
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投稿 Sep 15 2014

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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