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Fusion protein corresponding to Human SNX27 (N terminal).
Our Abpromise guarantee covers the use of ab77799 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 0.1-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||Use a concentration of 10 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Abcam recommends using 3% milk as the blocking agent.
|IP||Use at an assay dependent concentration.|
|ICC/IF||1/500. Use with paraformaldehyde- or methanol- fixed cells.|
Blocking: 5% Milk in TBS-T.
Two bands are observed very close together, at around 60-kDa. There are four reported isoforms of the SNX27 protein (Swissprot). We hypothesise that this antibody detects both isoform 1 and isoform 2, which are 61-kDa and 60-kDa respectively. As the immunogen is derived from the N-terminal region of SNX27, the antibody is not expected to react with isoforms 3 or 4.
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