Anti-SNX27 抗体 [1C6] (ab77799)
Key features and details
- Mouse monoclonal [1C6] to SNX27
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF
- Reacts with: Human
- Isotype: IgG1
Related conjugates and formulations
製品の概要
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製品名
Anti-SNX27 antibody [1C6]
SNX27 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [1C6] to SNX27 -
由来種
Mouse -
アプリケーション
適用あり: Flow Cyt (Intra), WB, IP, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Monkey非交差種: Mouse -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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エピトープ
N-terminal amino acids 1-267. -
ポジティブ・コントロール
- This antibody gave a positive signal in the following whole cell lysates: HeLa; A431; HEK293; A549; MDA-MB-231. ICC/IF: Jurkat cells
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特記事項
This antibody clone is manufactured by Abcam. If you require a custom buffer formulation or conjugation for your experiments, please contact orders@abcam.com.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 6.97% L-Arginine -
Concentration information loading...
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精製度
Protein G purified -
ポリ/モノ
モノクローナル -
クローン名
1C6 -
ミエローマ
Sp2/0 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab77799の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
Use 0.1-1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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WB | (6) |
Use a concentration of 10 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa).
Abcam recommends using 3% milk as the blocking agent. |
IP |
Use at an assay dependent concentration.
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ICC/IF | (4) |
Use a concentration of 5 µg/ml.
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特記事項 |
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Flow Cyt (Intra)
Use 0.1-1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
WB
Use a concentration of 10 µg/ml. Detects a band of approximately 61 kDa (predicted molecular weight: 61 kDa). Abcam recommends using 3% milk as the blocking agent. |
IP
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 5 µg/ml. |
ターゲット情報
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機能
Involved in endocytic trafficking (By similarity). In T lymphocytes, participates in endocytic recycling pathway. Recruits PSCDBP and HT4R to early endosomes. -
組織特異性
Expressed in cells of hematopoietic origin (at protein level). -
配列類似性
Belongs to the sorting nexin family.
Contains 1 PDZ (DHR) domain.
Contains 1 PX (phox homology) domain.
Contains 1 Ras-associating domain. -
ドメイン
The PDZ domain mediates the interaction with DGKZ, PSCDBP and HT4R and is responsible for vesicular localization. -
細胞内局在
Cytoplasm > cytosol. Early endosome. In T-lymphocytes, recruited from the cytosol to sorting endosomes by phosphoinositide-3-kinase products. - Information by UniProt
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参照データベース
- Entrez Gene: 81609 Human
- Omim: 611541 Human
- SwissProt: Q96L92 Human
- Unigene: 192326 Human
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別名
- KIAA0488 antibody
- Methamphetamine responsive transcript 1 antibody
- MGC126871 antibody
see all
画像
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All lanes : Anti-SNX27 antibody [1C6] (ab77799) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
Lane 4 : A549 (Human lung adenocarcinoma epithelial cell line) Whole Cell Lysate
Lane 5 : MDA-MB-231 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Mouse IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 61 kDa
Observed band size: 61 kDa
Exposure time: 15 minutesBlocking: 5% Milk in TBS-T.
Two bands are observed very close together, at around 60-kDa. There are four reported isoforms of the SNX27 protein (Swissprot). We hypothesise that this antibody detects both isoform 1 and isoform 2, which are 61-kDa and 60-kDa respectively. As the immunogen is derived from the N-terminal region of SNX27, the antibody is not expected to react with isoforms 3 or 4.
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ab77799 staining SNX27 in Jurkat cells. The cells were fixed with 100% methanol (5 min), permeabilized with 0.1% PBS-Tween for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at 4°C with ab77799 at 5µg/ml and ab6046, Rabbit polyclonal to beta Tubulin - Loading Control. Cells were then incubated with ab150117, Goat polyclonal Secondary Antibody to Mouse IgG H&L (Alexa Fluor® 488) preadsorbed at 1/1000 dilution (shown in green) and ab150080, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 594) at 1/1000 dilution (shown in pseudocolour magenta). Nuclear DNA was labelled with DAPI (shown in blue). Image was acquired with a confocal microscope (Leica-Microsystems TCS SP8) and a single confocal section is shown.
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Overlay histogram showing Jurkat cells stained with ab77799 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77799, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
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SNX27 was immunoprecipitated using 0.5mg Hek293 whole cell extract, 5µg of Mouse monoclonal to SNX27 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).
The antibody was incubated under agitation with Protein G beads for 10min, Hek293 whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.
Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab77799.
Secondary: Goat polyclonal to mouse IgG light chain specific (HRP) at 1/5000 dilution.
Band: 61kDa: SNX27.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (38)
ab77799 は 38 報の論文で使用されています。
- Yang B et al. SNX27 suppresses SARS-CoV-2 infection by inhibiting viral lysosome/late endosome entry. Proc Natl Acad Sci U S A 119:N/A (2022). PubMed: 35022217
- Simonetti B et al. SNX27-Retromer directly binds ESCPE-1 to transfer cargo proteins during endosomal recycling. PLoS Biol 20:e3001601 (2022). PubMed: 35417450
- González-Mancha N et al. Sorting Nexin 27 Enables MTOC and Secretory Machinery Translocation to the Immune Synapse. Front Immunol 12:814570 (2021). PubMed: 35095913
- Fokin AI et al. The Arp1/11 minifilament of dynactin primes the endosomal Arp2/3 complex. Sci Adv 7:N/A (2021). PubMed: 33523880
- Zhu Y et al. A genome-wide CRISPR screen identifies host factors that regulate SARS-CoV-2 entry. Nat Commun 12:961 (2021). PubMed: 33574281