The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1.25 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
May be involved in several stages of intracellular trafficking. Could play an important role in the regulation of glucose transport by insulin. May act as a downstream effector of RHOQ/TC10 in the regulation of insulin-stimulated glucose transport.
Belongs to the PX domain-containing GAP family. Contains 1 PX (phox homology) domain. Contains 1 Rho-GAP domain. Contains 1 SH3 domain.
ab53513 at 1.25 µg/ml staining human prostate by IHC-P. The sample underwent steamed antigen retrieval with citrate buffer (pH6) and AP staining. The image shows cytoplasm staining of secretory cells in the glands.