SNX1 overexpression 293T lysate (whole cell) (ab94159)

製品の概要

  • 製品名
    SNX1 overexpression 293T lysate (whole cell)
    SNX1 ライゼート 製品一覧
  • 製品の詳細
    SNX1 overexpression lysate
  • 特記事項
    ab94159 is a 293T cell transfected lysate in which Human SNX1 has been transiently over-expressed using a pCMV-SNX1 plasmid. The lysate is provided in 1X Sample Buffer. Note: For more details about how the transfected lysate was prepared view the preparation notes.
  • アプリケーション
    適用あり: WBmore details

法規制情報

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles.
  • バッファー
    pH: 6.80
    Constituent: 100% 1x Sample Buffer
  • Concentration information loading...
  • 精製度
    Whole Cell Lysate
  • 研究分野
  • 背景
    Function: May be involved in several stages of intracellular trafficking. Plays a role in targeting ligand-activated EGFR to the lysosomes for degradation after endocytosis from the cell surface and release from the Golgi. Component of the retromer complex, a complex required to retrieve lysosomal enzyme receptors (IGF2R and M6PR) from endosomes to the trans-Golgi network. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Similarity: Belongs to the sorting nexin family. Contains 1 BAR domain. Contains 1 PX (phox homology) domain.

アプリケーション

Our Abpromise guarantee covers the use of ab94159 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent dilution.

画像

  • ab94159 at 15 µg/lane on an SDS-PAGE gel
  • All lanes : Anti-SNX1 antibody (ab55776) at 1/500 dilution

    Lane 1 : SNX1 overexpression 293T lysate (whole cell) (ab94159)
    Lane 2 : 293T non-transfected Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution

参考文献

ab94159 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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