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Small nuclear ribonucleoprotein polypeptide G antibody
Western blot - Anti-SNRPG antibody (ab111194)
Anti-SNRPG antibody (ab111194) at 1 µg/ml (Milk blocking 3%) + HEK293 (Human) Whole Cell Lysate (ab52256) at 25 µg
Secondary Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution Developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 8 kDa Observed band size : 8 kDa Additional bands at : 36 kDa (possible non-specific binding),80 kDa (possible non-specific binding).
Exposure time : 90 seconds
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% Milk before being incubated with ab111194 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.