製品の概要

  • 製品名Anti-SNRP70 antibody
    SNRP70 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SNRP70
  • アプリケーション適用あり: IHC-P, ICC/IF, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
    交差が予測される動物種: Cow
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 400 to the C-terminus of Human SNRP70.

    (Peptide available as ab95039.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in Mouse Skeletal muscle and Mouse Heart tissue lysates as wellas the following whole cell lysates: HeLa; Jurkat; HepG2; HEK293; CaCo2; MCF7; SHSY5Y; NIH3T3; MEF1.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab83306 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P Use a concentration of 1 µg/ml.
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 51 kDa).

ターゲット情報

  • 関連性SNRP70 is an RNA-binding protein that is a specific component of the U1 small nuclear ribonucleoprotein complex and constitutes the major anti-(U1) RNP autoimmune antigen. SNRP70 contains 1 RRM (RNA recognition motif) domain and mediates the splicing of pre-mRNA by binding to the loop I region of U1-snRNA.
  • 細胞内局在Nuclear
  • 参照データベース
  • 別名
    • RNPU1Z antibody
    • Rnulp70 antibody
    • RPU1 antibody
    • Small nuclear ribonucleoprotein 70 (U1) antibody
    • Small nuclear ribonucleoprotein 70kD polypeptide (RNP antigen) antibody
    • Small nuclear ribonucleoprotein 70kDa (U1) antibody
    • Small nuclear ribonucleoprotein 70kDa polypeptide (RNP antigen) antibody
    • snRNP70 antibody
    • Snrp 70 antibody
    • SNRP70 antibody
    • U1 70K antibody
    • U1 small nuclear ribonucleoprotein 70 kDa antibody
    • U1 small nuclear ribonucleoprotein polypeptide A antibody
    • U1 snRNP 70 kDa antibody
    • U170K antibody
    • U1AP antibody
    • U1AP1 antibody
    • U1RNP antibody
    see all

Anti-SNRP70 antibody 画像

  • All lanes : Anti-SNRP70 antibody (ab83306) at 1 µg/ml

    Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
    Lane 2 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
    Lane 3 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
    Lane 4 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
    Lane 5 : Caco 2 (Human colonic carcinoma cell line) Whole Cell Lysate
    Lane 6 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 7 : SHSY-5Y (Human neuroblastoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)


    Exposure time : 2 minutes
    The 63 kDa band observed is comparable to the molecular weight seen with other commercially available antibodies to Human U1 small nuclear ribonucleoprotein 70 kDa.
  • ICC/IF image of ab83306 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab83306, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) HepG2, and Hek293 cells at 5µg/ml.
  • IHC image of Ab83306 staining in Human Cervical carcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with Ab83306, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-SNRP70 antibody (ab83306) at 1 µg/ml

    Lane 1 : Testis (Rat) Tissue Lysate
    Lane 2 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
    Lane 3 : Testis (Mouse) Tissue Lysate
    Lane 4 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 51 kDa
    Observed band size : 63 kDa (why is the actual band size different from the predicted?)
    Additional bands at : 24 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 1 minute

Anti-SNRP70 antibody (ab83306) 使用論文

This product has been referenced in:
  • Franco HL  et al. TNFa signaling exposes latent estrogen receptor binding sites to alter the breast cancer cell transcriptome. Mol Cell 58:21-34 (2015). Human . Read more (PubMed: 25752574) »
  • Mozzetti S  et al. Gli family transcription factors are drivers of patupilone resistance in ovarian cancer. Biochem Pharmacol 84:1409-18 (2012). Read more (PubMed: 22964220) »

See all 2 Publications for this product

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