製品の概要

  • 製品名Anti-SNRNP200 antibody
    SNRNP200 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SNRNP200
  • アプリケーション適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 212-261 (GDEDVYGEVR EEASDDDMEG DEAVVRCTLS ANLVASGELM SSKKKDLHPR) of Human SNRNP200 (NP_054733, O75643).

  • ポジティブ・コントロール
    • COLO205 lysate

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • バッファーConstituents: 97% PBS, 2% Sucrose
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab118713 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/200.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 244 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能Putative RNA helicase involved in the second step of RNA splicing. May promote one or more conformational changes in the dynamic network of RNA-RNA interactions in the spliceosome. Appears to catalyze an ATP-dependent unwinding of U4/U6 RNA duplices.
  • 組織特異性Widely expressed.
  • 関連疾患Defects in SNRNP200 are the cause of retinitis pigmentosa type 33 (RP33) [MIM:610359]. It is a retinal dystrophy belonging to the group of pigmentary retinopathies. Retinitis pigmentosa is characterized by retinal pigment deposits visible on fundus examination and primary loss of rod photoreceptor cells followed by secondary loss of cone photoreceptors. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well.
  • 配列類似性Belongs to the helicase family. SKI2 subfamily.
    Contains 2 helicase ATP-binding domains.
    Contains 2 helicase C-terminal domains.
    Contains 2 SEC63 domains.
  • ドメインComposed of two similar domains.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Activating signal cointegrator 1 complex subunit 3-like 1 antibody
    • ASCC3L1 antibody
    • BRR2 antibody
    • BRR2 homolog antibody
    • HELIC2 antibody
    • RP33 antibody
    • SNRNP200 antibody
    • U5 small nuclear ribonucleoprotein 200 kDa helicase antibody
    • U5 snRNP-specific 200 kDa protein antibody
    • U5-200KD antibody
    • U520_HUMAN antibody
    see all

Anti-SNRNP200 antibody 画像

  • Anti-SNRNP200 antibody (ab118713) at 1 µg/ml + COLO205 lysate at 10 µg

    Secondary
    Goat anti-Rabbit IgG-HRP

    Predicted band size : 244 kDa

Anti-SNRNP200 antibody (ab118713) 使用論文

This product has been referenced in:
  • Liu YC  et al. Cytoplasmic Viral RNA-Dependent RNA Polymerase Disrupts the Intracellular Splicing Machinery by Entering the Nucleus and Interfering with Prp8. PLoS Pathog 10:e1004199 (2014). WB ; Human . Read more (PubMed: 24968230) »

See 1 Publication for this product

Product Wall

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (HeLa S3)
Specification HeLa S3
Fixative Formaldehyde
Permeabilization Yes - Triton 0.3%
Blocking step Donkey Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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Verified customer

投稿 Nov 05 2012

Thank you for your reply. ICC/IF has not been tested, thus, you are eligible to use our testing discount program.

DISCOUNT CODE: xxx
Expiration date: xxx

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Thank you for contacting us.

Yes, you can definitely use our testing discount program to try the antibody in IF.

Could you please let me know what type of IF you would like to use it in: ICC/IF (with cultured cells) or IHC-P (paraf...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"