Anti-SNPH 抗体 (ab69992)
Key features and details
- Rabbit polyclonal to SNPH
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-SNPH antibody
SNPH 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SNPH -
由来種
Rabbit -
アプリケーション
適用あり: ICC/IF, IHC-P, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide within Human SNPH aa 80-130 (N terminal). The exact sequence is proprietary.
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ポジティブ・コントロール
- Human brain tissue lysate
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab69992の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ICC/IF |
Use a concentration of 20 µg/ml.
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IHC-P |
Use a concentration of 5 µg/ml.
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WB |
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 58 kDa).
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特記事項 |
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ICC/IF
Use a concentration of 20 µg/ml. |
IHC-P
Use a concentration of 5 µg/ml. |
WB
Use a concentration of 2 - 4 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 58 kDa). |
ターゲット情報
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機能
Inhibits SNARE complex formation by absorbing free syntaxin-1. -
組織特異性
Brain specific. Found in synapses. -
細胞内局在
Membrane. Cell junction > synapse > synaptosome. - Information by UniProt
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参照データベース
- Entrez Gene: 9751 Human
- Omim: 604942 Human
- SwissProt: O15079 Human
- Unigene: 323833 Human
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別名
- bA314N13.5 antibody
- KIAA0374 antibody
- Snph antibody
see all
画像
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Lane 1 : Anti-SNPH antibody (ab69992) at 2 µg/ml
Lane 2 : Anti-SNPH antibody (ab69992) at 4 µg/ml
All lanes : Human brain tissue lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 58 kDa
Observed band size: 75 kDa why is the actual band size different from the predicted?
Additional bands at: 115 kDa, 120 kDa, 57 kDa, 65 kDa, 70 kDa, 84 kDa. We are unsure as to the identity of these extra bands. -
ab69992 at 5 µg/ml staining SNPH in human brain tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).
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Immunofluorescence of Syntaphilin in Human Brain cells using ab69992 at 20 ug/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab69992 は 1 報の論文で使用されています。
- Feng Y et al. Dl-3-n-Butylphthalide Alleviates Demyelination and Improves Cognitive Function by Promoting Mitochondrial Dynamics in White Matter Lesions. Front Aging Neurosci 13:632374 (2021). PubMed: 33762923