製品の概要

  • 製品名Anti-SNF5 antibody
    SNF5 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SNF5
  • 特異性This antibody is specific for SMARCB1.
  • アプリケーション適用あり: ICC/IF, ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rat, Horse, Guinea pig, Cow, Dog
  • 免疫原

    A region within synthetic peptide: RGSLYKRYPS LWRRLATVEE RKKIVASSHG KKTKPNTKDH GYTTLATSVT, corresponding to amino acids 40-89 of Human SNF5

  • ポジティブ・コントロール
    • Human liver tissue and fetal thymus lysates.

製品の特性

関連製品

アプリケーション

Our Abpromise guarantee covers the use of ab42503 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF 1/150.
ELISA 1/312500.
WB Use a concentration of 2 µg/ml. Detects a band of approximately 44 kDa (predicted molecular weight: 44 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
IHC-P Use a concentration of 4 - 8 µg/ml.

ターゲット情報

  • 機能Core component of the BAF (hSWI/SNF) complex. This ATP-dependent chromatin-remodeling complex plays important roles in cell proliferation and differentiation, in cellular antiviral activities and inhibition of tumor formation. The BAF complex is able to create a stable, altered form of chromatin that constrains fewer negative supercoils than normal. This change in supercoiling would be due to the conversion of up to one-half of the nucleosomes on polynucleosomal arrays into asymmetric structures, termed altosomes, each composed of 2 histones octamers. Stimulates in vitro the remodeling activity of SMARCA4/BRG1/BAF190A. Involved in activation of CSF1 promoter. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a post-mitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to post-mitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth (By similarity). Plays a key role in cell-cycle control and causes cell cycle arrest in G0/G1. Also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene.
  • 関連疾患Defects in SMARCB1 are a cause of rhabdoid tumor (RDT) [MIM:609322]; also known as malignant rhabdoid tumor (MRT). RDT are a highly malignant group of neoplasms that usually occur in early childhood. SMARCB1/INI1 is also frequently inactivated in epithelioid sarcomas.
    Defects in SMARCB1 are a cause of schwannomatosis (SCHWA) [MIM:162091]; also called congenital cutaneous neurilemmomatosis. Schwannomas are benign tumors of the peripheral nerve sheath that usually occur singly in otherwise normal individuals. Multiple schwannomas in the same individual suggest an underlying tumor-predisposition syndrome. The most common such syndrome is NF2. The hallmark of NF2 is the development of bilateral vestibular-nerve schwannomas; but two-thirds or more of all NF2-affected individuals develop schwannomas in other locations, and dermal schwannomas may precede vestibular tumors in NF2-affected children. There have been several reports of individuals with multiple schwannomas who do not show evidence of vestibular schwannoma. Clinical report suggests that schwannomatosis is a clinical entity distinct from other forms of neurofibromatosis.
  • 配列類似性Belongs to the SNF5 family.
  • 翻訳後修飾Phosphorylated upon DNA damage, probably by ATM or ATR.
  • 細胞内局在Nucleus.
  • Information by UniProt
  • 参照データベース
  • 別名
    • BAF47 antibody
    • BRG1-associated factor 47 antibody
    • hSNF5 antibody
    • INI1 antibody
    • Integrase interactor 1 protein antibody
    • Malignant rhabdoid tumor suppressor antibody
    • RDT antibody
    • RTPS1 antibody
    • Sfh1p antibody
    • SMARCB1 antibody
    • SNF5 homolog antibody
    • SNF5_HUMAN antibody
    • SNF5L1 antibody
    • Snr1 antibody
    • Sucrose nonfermenting yeast homolog like 1 antibody
    • SWI/SNF complex component SNF5 antibody
    • SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily B member 1 antibody
    • SWI10 antibody
    • Transcription factor TYE4 antibody
    • Transcription regulatory protein SNF5 antibody
    • TYE4 antibody
    see all

Anti-SNF5 antibody 画像

  • Anti-SNF5 antibody (ab42503) at 2 µg/ml + fetal thymus lysate

    Secondary
    HRP conjugated anti-Rabbit IgG at 1/50000 dilution

    Predicted band size : 44 kDa
    Observed band size : 44 kDa
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human liver tissue labelling SNF5 with ab42503 at 4-8µg/ml.

  • ab42503 staining SNF5 in Mouse 3T3 cultured cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.5% Triton X 100 and blocked with 5% serum for 30 minutes. Samples were incubated with primary antibody (1/150) for 16 hours at 4°C. An Alexa Fluor®546-conjugated goat anti-rabbit IgG polyclonal (1/2000) was used as the secondary antibody.

    See Abreview

Anti-SNF5 antibody (ab42503) 使用論文

ab42503 has not yet been referenced specifically in any publications.

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Thank you for contacting us and sorry for the delay in getting back to you. The following protocol was used to perform the staining presented on the datasheet of the anti-SNF5 antibody (ab42503):

Tissue fixation and preparation

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It is clear that ab42503 does not detect SNF5, it may well be that it has been damaged acci...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Immunocytochemistry/ Immunofluorescence
Sample Mouse Cultured Cells (3T3)
Specification 3T3
Fixative Formaldehyde
Permeabilization Yes - 0.5% triton X100
Blocking step Serum as blocking agent for 30 minute(s) · Concentration: 5%
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投稿 Apr 22 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"