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Our Abpromise guarantee covers the use of ab3749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 16917504|
|ChIP||Use 2.5µl for 106 cells.|
|WB||1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).|
Rabbit polyclonal ab3749 to SNF2H was used in all lanes at 1/500 dilution.
Lane 1: HeLa lysate (20ug)
Lane 2: Mouse 3T3 lysate (20ug)
Lane 3: HeLa lysate (20ug), blocking peptide (ab5160) 1.0ug
Lane 4: Mouse 3T3 lysate (20ug), blocking peptide (ab5160) 1.0ug
Secondary antibody: Goat polyclonal to Rabbit IgG (ab6721)
ab3749 was able to recognise SNF2H in HeLA and Mouse 3T3 lysates (band at ~120 kDa). Binding was blocked by the immunising peptide ab5160.
ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estro