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Our Abpromise guarantee covers the use of ab3749 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration. PubMed: 16917504|
|ChIP||Use 2.5µl for 106 cells.|
|WB||1/500. Detects a band of approximately 120 kDa (predicted molecular weight: 122 kDa).|
ab3749 works in ChIP, as shown by the detection of an increase in the recruitment of SNF2H to the estrogen-responsive pS2 promoter.
Sonicated Chromatin prepared from untreated or 17beta-estradiol (E) treated MCF7 cells was subjected to the ChIP procedure with ab3749 antibody specific to SNF2H. The immunoprecipitated chromatin was analysed in the proximal region of the estrogen-responsive pS2 promoter (as shown above) and quantified by real-time PCR (values are % of inputs). The primers were designed to follow the nucleosome E (including the Estrogen Responsive Element ERE). 2.5