SNAP25 overexpression 293T lysate (whole cell) (ab94226)
製品の概要
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製品名
SNAP25 overexpression 293T lysate (whole cell)
SNAP25 ライゼート 製品一覧 -
特記事項
ab94226 is a 293T cell transfected lysate in which Human SNAP25 has been transiently over-expressed using a pCMV-SNAP25 plasmid. The lysate is provided in 1X Sample Buffer.
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アプリケーション
適用あり: WBmore details
製品の特性
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Mycoplasma free
Yes -
製品の状態
Liquid -
保存方法
Shipped on dry ice. Upon delivery aliquot and store at -20ºC. Avoid freeze / thaw cycles. -
バッファー
Constituents: 0.01% Bromophenol blue, 2.3% Beta mercaptoethanol, 2% Sodium lauryl sulfate, 0.788% Tris HCl, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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研究分野
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背景
Function: t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF. Tissue specificity: Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum. Similarity: Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains. PTM: Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
関連製品
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Corresponding Antibody
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab94226の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use at an assay dependent dilution.
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特記事項 |
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WB
Use at an assay dependent dilution. |
画像
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ab94226 at 15μg/lane on an SDS-PAGE gel
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All lanes : Anti-SNAP25 antibody [4A3] (ab66066) at 1/500 dilution
Lane 1 :SNAP25 overexpression 293T lysate (whole cell) (ab94226)
Lane 2 : 293T Non Transfected Lysate
Lysates/proteins at 25 µg per lane.
Secondary
All lanes : Goat Anti-mouse IgG (H and L) HRP conjugated at 1/2500 dilution
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab94226 は論文での使用が確認できていません。