特記事項This product should be stored undiluted.
Storage in frost free freezers is not recommended.
Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of despatch.
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
追加情報ELISA: Use at an assay dependent dilution.
IHC-P: 1/2000 - 1/5000. This product does not require protein digestion pre-treatment or antigen retrieval using heat treatment prior to staining of paraffin sections.
WB: 1/500 - 1/5000. Detects a band of approximately 26-27 kDa.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
機能t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
組織特異性Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
配列類似性Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains.
翻訳後修飾Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
細胞内局在Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.