The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 25 kDa (predicted molecular weight: 23 kDa).
1/100 - 1/250. Antigen retrieval is recommended.
1/100 - 1/250.
Is unsuitable for Flow Cyt or IP.
t-SNARE involved in the molecular regulation of neurotransmitter release. May play an important role in the synaptic function of specific neuronal systems. Associates with proteins involved in vesicle docking and membrane fusion. Regulates plasma membrane recycling through its interaction with CENPF.
Neurons of the neocortex, hippocampus, piriform cortex, anterior thalamic nuclei, pontine nuclei, and granule cells of the cerebellum.
Belongs to the SNAP-25 family. Contains 2 t-SNARE coiled-coil homology domains.
Palmitoylated. Cys-85 appears to be the main site, and palmitoylation is required for membrane association.
Cytoplasm > perinuclear region. Cell membrane. Cell junction > synapse > synaptosome. Membrane association requires palmitoylation. Expressed throughout cytoplasm, concentrating at the perinuclear region.
Shi TY et al. Kainate receptor mediated presynaptic LTP in agranular insular cortex contributes to fear and anxiety in mice. Neuropharmacology128:388-400 (2018).
Read more (PubMed: 29104074) »
Almandoz-Gil L et al. In Situ Proximity Ligation Assay Reveals Co-Localization of Alpha-Synuclein and SNARE Proteins in Murine Primary Neurons. Front Neurol9:180 (2018).
Read more (PubMed: 29623065) »