製品の概要

  • 製品名Anti-SNAIL antibody
    SNAIL 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SNAIL
  • アプリケーション適用あり: Flow Cyt, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    3 synthetic peptides (human) conjugated to KLH

  • ポジティブ・コントロール
    • This antibody gave a positive signal when tested against the SNAIL recombinant protein; Primary human keratinocyte cell lysate; Induced culture of E.coli (one shot Top10 competent cells).

法規制情報

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82846 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt Use at an assay dependent concentration. PubMed: 24932263

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

WB 1/200 - 1/500. Detects a band of approximately 29 kDa (predicted molecular weight: 29 kDa).

ターゲット情報

  • 機能Involved in induction of the epithelial to mesenchymal transition (EMT), formation and maintenance of embryonic mesoderm, growth arrest, survival and cell migration. Binds to 3 E-boxes of the E-cadherin/CDH1 gene promoter and to the promoters of CLDN7 and KRT8 and, in association with histone demethylase KDM1A which it recruits to the promoters, causes a decrease in dimethylated H3K4 levels and represses transcription. Associates with EGR1 and SP1 to mediate tetradecanoyl phorbol acetate (TPA)-induced up-regulation of CDKN2B, possibly by binding to the CDKN2B promoter region 5'-TCACA-3. In addition, may also activate the CDKN2B promoter by itself.
  • 組織特異性Expressed in a variety of tissues with the highest expression in kidney. Expressed in mesenchymal and epithelial cell lines.
  • 配列類似性Belongs to the snail C2H2-type zinc-finger protein family.
    Contains 4 C2H2-type zinc fingers.
  • 翻訳後修飾Phosphorylated by GSK3B. Once phosphorylated, it becomes a target for BTRC ubiquitination. Phosphorylation by CSNK1E, probably at Ser-104, provides the priming site for the subsequent phosphorylation by GSK3B, probably at Ser-100 and Ser-96. Phosphorylation by PAK1 may modulate its transcriptional activity by promoting increased accumulation in the nucleus. Phosphorylation at Ser-11 and Ser-92 positively regulates its functions in induction of EMT and cell survival, respectively. Phosphorylation by LATS2, upon mitotic stress, oncogenic stress or Hippo pathway activation, occurs in the nucleus and promotes nuclear retention and stabilization of total cellular protein level.
    Ubiquitinated on Lys-98, Lys-137 and Lys-146 by FBXL14 and BTRC leading to degradation. BTRC-triggered ubiquitination requires previous GSK3B-mediated SNAI1 phosphorylation. Ubiquitination induced upon interaction with NOTCH1 or TP53/p53 is mediated by MDM2.
    O-GlcNAcylation at Ser-112 is enhanced in hyperglycaemic conditions, it opposes phosphorylation by GSK3B, and stabilizes the protein.
    ADP-ribosylation by PARP1 increases protein half-life and may be involved in TGFB-induced SNAI1 up-regulation.
  • 細胞内局在Nucleus. Cytoplasm. Once phosphorylated (probably on Ser-107, Ser-111, Ser-115 and Ser-119) it is exported from the nucleus to the cytoplasm where subsequent phosphorylation of the destruction motif and ubiquitination involving BTRC occurs.
  • Information by UniProt
  • 参照データベース
  • 別名
    • dJ710H13.1 antibody
    • Protein sna antibody
    • Protein snail homolog 1 antibody
    • Protein snail homolog antibody
    • SLUGH2 antibody
    • SNA antibody
    • Sna protein antibody
    • SNAH antibody
    • SNAI antibody
    • snai1 antibody
    • SNAI1_HUMAN antibody
    • Snail 1 homolog antibody
    • Snail 1 zinc finger protein antibody
    • SNAIL antibody
    • Snail homolog 1 (Drosophila) antibody
    • SNAIL, Drosophila, homolog of, 1 antibody
    • SNAIL1 antibody
    • Zinc finger protein SNAI1 antibody
    see all

Anti-SNAIL antibody 画像

  • All lanes : Anti-SNAIL antibody (ab82846) at 1/200 dilution

    Lane 1 : SNAIL (SNAI1) Human Recombinant Protein
    Lane 2 : SLUG (SNAI2) Human Recombinant Protein

    Lysates/proteins at 0.1 µg per lane.

    Secondary
    Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 29 kDa
    Observed band size : 68 kDa (why is the actual band size different from the predicted?)


    Exposure time : 1 minute
    Ab82846 recognizes the tagged recombinant SNAIL protein which has an expected molecular weight of 68 kDa
  • All lanes : Anti-SNAIL antibody (ab82846) at 1/200 dilution

    Lane 1 : Primary human keratinocyte cell lysate (normal)
    Lane 2 : Primary human keratinocyte cell lysate (upregulated)

    Lysates/proteins at 30 µg per lane.

    Secondary
    anti-Rabbit IgG HRP at 1/1000 dilution

    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 16 kDa,60 kDa. We are unsure as to the identity of these extra bands.
  • All lanes : Anti-SNAIL antibody (ab82846) at 1/500 dilution

    Lane 1 : uninduced culture of E.coli (one shot Top10 competent cells)
    Lane 2 : induced culture of E.coli (one shot Top10 competent cells)


    Predicted band size : 29 kDa
    Observed band size : 29 kDa
    Additional bands at : 65 kDa. We are unsure as to the identity of these extra bands.
    Plasmid name: pBAD-DEST49.
    Molecular weight:43.1kDa (29.1kDa + 14kDa for the tag).

Anti-SNAIL antibody (ab82846) 使用論文

This product has been referenced in:
  • Abu N  et al. Flavokawain B induced cytotoxicity in two breast cancer cell lines, MCF-7 and MDA-MB231 and inhibited the metastatic potential of MDA-MB231 via the regulation of several tyrosine kinases In vitro. BMC Complement Altern Med 16:86 (2016). WB . Read more (PubMed: 26922065) »
  • Song J  et al. Hepatic stellate cells activated by acidic tumor microenvironment promote the metastasis of hepatocellular carcinoma via osteopontin. Cancer Lett 356:713-20 (2015). Read more (PubMed: 25449435) »

See all 6 Publications for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Blocking step Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: RT°C
Sample Mouse Cell (MSC)
Specification MSC
Permeabilization Yes - Triton 1%
Fixative Formaldehyde
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投稿 Feb 23 2015

Thank you for contacting us.

A good positive control would be ab114553, human SNAIL protein.

http://www.abcam.com/SNAIL-protein-ab114553.html

I hope this information is helpful to you. Please do not hesitate to contact u...

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Es tut mir leid, dass Sie Probleme mit diesem Antikörper hatten. Wie am Telefon besprochen habe ich eine kostenlose Ersatzlieferung für Sie in Auftrag gegeben. Sie hat die Referenznummer XXXXX und sollte ab dem 23. Mai bei Ihnen ankommen.

Al...

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Thanks for contacting us with your question. The rabbit SNAIL antibody ab82846 was not produced with the same immunogen as ab85931. Ab82846 has been tested for cross-reactivity with recombinant SLUG in Western blot and there is no cross-reactivity (see...

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