Rabbit polyclonal to SMYD5
Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Drosophila melanogaster, Zebrafish
Synthetic peptide corresponding to a region within C terminal amino acids 360-409 (SRHSRHKILR ENYLFVCSCP KCLAEADEPN VTSEEEEEEE EEEEGEPEDA) of human SMYD5 (NP_006053).
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Constituents: 2% Sucrose, PBS
Concentration information loading...
Immunogen affinity purified
Purified by peptide affinity chromatography method.
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.
Use a concentration of 5 µg/ml.
Use at an assay dependent dilution.
Contains 1 MYND-type zinc finger.
Contains 1 SET domain.
Information by UniProt
Protein NN8 4AG antibody
Protein NN8-4AG antibody
Retinoic acid induced protein 15 antibody
Western blot - Anti-SMYD5 antibody (ab81419)
Anti-SMYD5 antibody (ab81419) at 1 µg/ml (in 5% skim milk / PBS buffer) + Hela cell lysate at 10 µg
HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size:
Observed band size:
42 and 47 kDa (
why is the actual band size different from the predicted?
Gel concentration: 12%
Immunocytochemistry/ Immunofluorescence - Anti-SMYD5 antibody (ab81419)
ICC/IF image of ab81419 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab81419, 5µg/ml) overnight at +4°C. The secondary antibody (green) was
Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
has not yet been referenced specifically in any publications.
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