製品の概要

  • 製品名Anti-SMURF1 antibody
    SMURF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SMURF1
  • アプリケーション適用あり: ELISA, WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    KLH-conjugated synthetic peptide selected from the N terminal region of human SMURF1 within aa 1~100.

  • ポジティブ・コントロール
    • Mouse kidney tissue lysate, human breast carcinoma tissue.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab38866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
WB 1/100 - 1/500. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
IHC-P 1/50 - 1/100.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • 翻訳後修飾Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase SMURF1 antibody
    • hSMURF1 antibody
    • KIAA1625 antibody
    • Smad specific E3 ubiquitin ligase 1 antibody
    • SMAD specific E3 ubiquitin protein ligase 1 antibody
    • Smad ubiquitination regulatory factor 1 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 1 antibody
    • SMUF1_HUMAN antibody
    • SMURF 1 antibody
    • smurf1 antibody
    see all

Anti-SMURF1 antibody 画像

  • Anti-SMURF1 antibody (ab38866) at 1/100 dilution + mouse kidney tissue lysate at 35 µg

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
  • ab22983 at a 1/50 dilution, staining human breast carcinoma by immunohistochemistry by formalin-fixed and paraffin-embedded tissue, followed by AEC staining.

Anti-SMURF1 antibody (ab38866) 使用論文

This product has been referenced in:
  • Vimalraj S  et al. A positive role of microRNA-15b on regulation of osteoblast differentiation. J Cell Physiol 229:1236-44 (2014). Read more (PubMed: 24435757) »
  • van Dekken H  et al. Array comparative genomic hybridization, expression array, and protein analysis of critical regions on chromosome arms 1q, 7q, and 8p in adenocarcinomas of the gastroesophageal junction. Cancer Genet Cytogenet 189:37-42 (2009). Read more (PubMed: 19167610) »

See all 3 Publications for this product

Product Wall

Abreviews
Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (heads)
Loading amount 30 µg
Specification heads
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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Abcam user community

Verified customer

投稿 May 17 2012

Thank you for your call.

I have issued 2 discount codes as part of our "100% testing discount offer". To redeem this offer, please submit your Abreview with the Zebrafish data and include these codes in the "Additional Comments" section so t...

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (aortic smooth muscle cells)
Loading amount 20 µg
Specification aortic smooth muscle cells
Gel Running Conditions Reduced Denaturing (Bis-Tris 4-12% MOPS running buffer)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Username

Abcam user community

Verified customer

投稿 Apr 09 2008

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"