Anti-SMURF1 抗体 (ab115601)

製品の概要

  • 製品名Anti-SMURF1 antibody
    SMURF1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to SMURF1
  • アプリケーション適用あり: WB, IHC-Pmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    Synthetic peptide conjugated to KLH, corresponding to N terminal amino acids of Human SMURF1 (NP_851994.1).

  • ポジティブ・コントロール
    • Human testis tissue; Mouse kidney tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab115601 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Predicted molecular weight: 86 kDa.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

ターゲット情報

  • 機能E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway. Acts by mediating ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway. Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members.
  • パスウェイProtein modification; protein ubiquitination.
  • 配列類似性Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 2 WW domains.
  • 翻訳後修飾Ubiquitinated by the SCF(FBXL15) complex at Lys-381 and Lys-383, leading to its degradation by the proteasome. Lys-383 is the primary ubiquitination site.
  • 細胞内局在Cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase SMURF1 antibody
    • hSMURF1 antibody
    • KIAA1625 antibody
    • Smad specific E3 ubiquitin ligase 1 antibody
    • SMAD specific E3 ubiquitin protein ligase 1 antibody
    • Smad ubiquitination regulatory factor 1 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 1 antibody
    • SMUF1_HUMAN antibody
    • SMURF 1 antibody
    • smurf1 antibody
    see all

Anti-SMURF1 antibody 画像

  • ab115601, at 10µg/ml, staining SMURF1 in Formalin-fixed, Paraffin-embedded Human Testis tissue by Immunohistochemistry followed by biotinylated secondary antibody, alkaline phosphatase-streptavidin and chromogen.
  • Anti-SMURF1 antibody (ab115601) at 1/100 dilution + Mouse kidney lysate at 35 µg

    Predicted band size : 86 kDa

Anti-SMURF1 antibody (ab115601) 使用論文

ab115601 has not yet been referenced specifically in any publications.

Product Wall

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Zebrafish Tissue lysate - whole (heads)
Loading amount 30 µg
Specification heads
Gel Running Conditions Reduced Denaturing (10% gel)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 4°C
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投稿 May 17 2012

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"