製品の概要

  • 製品名Anti-SMURF 2 antibody [EP629Y3]
    SMURF 2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit monoclonal [EP629Y3] to SMURF 2
  • アプリケーション適用あり: WBmore details
    適用なし: Flow Cyt,ICC,IHC-P or IP
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    A synthetic peptide corresponding to residues in human Smurf2.

  • ポジティブ・コントロール
    • SH-SY-5Y cell lysate
  • 特記事項

    This product is a recombinant rabbit monoclonal antibody.

     

    Produced using Abcam’s RabMAb® technology. RabMAb® technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
  • 追加情報Is unsuitable for Flow Cyt,ICC,IHC-P or IP.
  • ターゲット情報

    • 機能E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
    • 組織特異性Widely expressed.
    • パスウェイProtein modification; protein ubiquitination.
    • 配列類似性Contains 1 C2 domain.
      Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
      Contains 3 WW domains.
    • ドメインThe second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
      The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
    • 翻訳後修飾Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
    • 細胞内局在Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
    • Information by UniProt
    • 参照データベース
    • 別名
      • E3 ubiquitin-protein ligase SMURF2 antibody
      • EC 6.3.2. antibody
      • hSMURF2 antibody
      • MGC138150 antibody
      • Smad specific E3 ubiquitin ligase 2 antibody
      • SMAD specific E3 ubiquitin protein ligase 2 antibody
      • SMAD ubiquitination regulatory factor 2 antibody
      • SMAD-specific E3 ubiquitin-protein ligase 2 antibody
      • SMUF2_HUMAN antibody
      • Smurf2 antibody
      • Ubiquitin protein ligase SMURF2 antibody
      see all

    Anti-SMURF 2 antibody [EP629Y3] 画像

    • Anti-SMURF 2 antibody [EP629Y3] (ab53316) at 1/500 dilution + SH-SY-5Y cell lysate at 10 µg

      Secondary
      Goat anti-rabbit HRP labeled at 1/2000 dilution

      Predicted band size : 86 kDa
      Observed band size : 86 kDa

    Anti-SMURF 2 antibody [EP629Y3] (ab53316) 使用論文

    This product has been referenced in:
    • Maxfield KE  et al. Comprehensive functional characterization of cancer-testis antigens defines obligate participation in multiple hallmarks of cancer. Nat Commun 6:8840 (2015). Read more (PubMed: 26567849) »
    • Wang M  et al. ATR/Chk1/Smurf1 pathway determines cell fate after DNA damage by controlling RhoB abundance. Nat Commun 5:4901 (2014). Read more (PubMed: 25249323) »

    See all 3 Publications for this product

    Product Wall

    Application Western blot
    Sample Mouse Cell lysate - whole cell (mouse liver cell line)
    Loading amount 15 µg
    Specification mouse liver cell line
    Gel Running Conditions Reduced Denaturing (8%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
    Username

    Dr. Frank Götschel

    Verified customer

    投稿 Sep 09 2009

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"