製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SMURF 2
  • 由来種
    Rabbit
  • 特異性
    This antibody is specific for SMURF 2 (N term).
  • アプリケーション
    適用あり: ELISA, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Human
  • 免疫原

    A KLH conjugated synthetic peptide (10-30 aa in length) at the N-term of first 50 aa of human SMURF 2.

  • ポジティブ・コントロール
    • Mouse brain tissue lysate and breast carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38543 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ELISA 1/1000.
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).

ターゲット情報

  • 機能
    E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Interacts with SMAD1 and SMAD7 in order to trigger their ubiquitination and proteasome-dependent degradation. In addition, interaction with SMAD7 activates autocatalytic degradation, which is prevented by interaction with SCYE1. Forms a stable complex with the TGF-beta receptor-mediated phosphorylated SMAD2 and SMAD3. In this way, SMAD2 may recruit substrates, such as SNON, for ubiquitin-mediated degradation. Enhances the inhibitory activity of SMAD7 and reduces the transcriptional activity of SMAD2. Coexpression of SMURF2 with SMAD1 results in considerable decrease in steady-state level of SMAD1 protein and a smaller decrease of SMAD2 level.
  • 組織特異性
    Widely expressed.
  • パスウェイ
    Protein modification; protein ubiquitination.
  • 配列類似性
    Contains 1 C2 domain.
    Contains 1 HECT (E6AP-type E3 ubiquitin-protein ligase) domain.
    Contains 3 WW domains.
  • ドメイン
    The second and third WW domains are responsible for interaction with the PY-motif of R-SMAD (SMAD1, SMAD2 and SMAD3).
    The C2 domain is involved in autoinhibition of the catalytic activity by interacting with the HECT domain.
  • 翻訳後修飾
    Auto-ubiquitinated and ubiquitinated in the presence of RNF11 and UBE2D1.
  • 細胞内局在
    Nucleus. Cytoplasm. Cell membrane. Membrane raft. Cytoplasmic in the presence of SMAD7. Co-localizes with CAV1, SMAD7 and TGF-beta receptor in membrane rafts.
  • Information by UniProt
  • 参照データベース
  • 別名
    • E3 ubiquitin-protein ligase SMURF2 antibody
    • EC 6.3.2. antibody
    • hSMURF2 antibody
    • MGC138150 antibody
    • Smad specific E3 ubiquitin ligase 2 antibody
    • SMAD specific E3 ubiquitin protein ligase 2 antibody
    • SMAD ubiquitination regulatory factor 2 antibody
    • SMAD-specific E3 ubiquitin-protein ligase 2 antibody
    • SMUF2_HUMAN antibody
    • Smurf2 antibody
    • Ubiquitin protein ligase SMURF2 antibody
    see all

画像

  • Anti-SMURF 2 antibody (ab38543) at 1/100 dilution + Mouse brain tissue lysate

    Predicted band size: 86 kDa
    Observed band size: 86 kDa

  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38543 at a 1/50 dilution, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining.

    This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.

参考文献

This product has been referenced in:
  • Itman C  et al. TGFß superfamily signaling regulators are differentially expressed in the developing and adult mouse testis. Spermatogenesis 1:63-72 (2011). Read more (PubMed: 21866277) »
  • Chen YL  et al. Smad6 inhibits the transcriptional activity of Tbx6 by mediating its degradation. J Biol Chem 284:23481-90 (2009). WB ; Mouse . Read more (PubMed: 19561075) »

See all 3 Publications for this product

レビューと Q&A

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Berast Cancer)
Loading amount
100000 cells
Specification
Berast Cancer
Gel Running Conditions
Reduced Denaturing (12.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Username

Ohad Tarcic

Verified customer

投稿 Dec 03 2012

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Oocyte)
Specification
Oocyte
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% Triton-x
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Username

CHEN Lei

Verified customer

投稿 Jun 20 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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