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Our Abpromise guarantee covers the use of ab17919 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent dilution.|
|WB||1/1000. Predicted molecular weight: 86 kDa. A nonspecific band is also observed at ~50kDa in HepG2 lysates.|
ab17919 staining SMURF 2 in Xenopus Laevis cultured retinal ganglion cell growth cone tissue by Immunocytochemistry/ Immunofluorescence. Cells were PFA-fixed and permeabilized in 0.1% Triton X-100 prior to blocking in 5% serum for 1 hour at 21°C. The primary antibody was diluted 1/250 in PBS and incubated with the sample for 16 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/1000.
ab17919 has not yet been referenced specifically in any publications.