The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
関連性Plays a role in the regulation of contractile properties of both striated and smooth muscles. When unphosphorylated, may inhibit myosin dephosphorylation. Phosphorylation at Ser-299 reduces this inhibitory activity.
細胞内局在Cytoplasm › myofibril. Cytoplasm › myofibril › sarcomere › I band By similarity. Cytoplasm › myofibril › sarcomere › M line. Nucleus. Note: Colocalizes with MYH2. In its unphosphorylated state, localizes to the cytoplasm. Phosphorylation at Ser-301 promotes translocation to the nucleus