製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to Smt3
  • 特異性
    ab14405 recognizes SUMO-GFP fusion proteins and purified Smt3. It is predicted to bind to yeast SUMO (SMT) bound to other proteins. Cross reactivity with other homologs may occur but has not been specifically tested.
  • アプリケーション
    適用あり: WB, ELISAmore details
  • 種交差性
    交差種: Saccharomyces cerevisiae
  • 免疫原

    Recombinant protein (S. cerevisiae).

  • ポジティブ・コントロール
    • Most yeast cell lysates can be used as a positive control without induction or stimulation.
  • 特記事項
    Centrifuge product if not completely clear after standing at room temperature.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.01% Sodium Azide
    Constituents: 0.15M Sodium chloride, 0.02M Potassium phosphate. pH 7.2
  • Concentration information loading...
  • 精製度
    IgG fraction
  • 特記事項(精製)
    This product is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit serum.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab14405 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/1000. Detects a band of approximately 12 kDa.
Note: Although not tested, this antibody is likely functional in Immunohistochemistry and Immunoprecipitation.
ELISA 1/1000 - 1/5000.

ターゲット情報

  • 機能
    Not known; suppressor of MIF2 mutations.
  • 配列類似性
    Belongs to the ubiquitin family. SUMO subfamily.
    Contains 1 ubiquitin-like domain.
  • Information by UniProt
  • 参照データベース
    • 別名
      • DmSUMO 1 antibody
      • Small Ubiquitin-like modifier antibody
      • SMT3 antibody
      • SMT3_YEAST antibody
      • Ubiquitin like protein of the SUMO family antibody
      • Ubiquitin like protein SMT3 antibody
      • Ubiquitin-like protein SMT3 antibody
      see all

    画像

    • All lanes : Anti-Smt3 antibody (ab14405) at 1/1000 dilution

      Lane 1 : 3 : 5 : 7 : 9 : 11 : 13, Lysate prepared from E.coli expressed Yeast SUMO protein and SUMO-GFP fusion protein after incubation with lysed insect cells
      Lane 2 : 4 : 6 : 8 : 10 : 12 : 14, Lysate prepared from SUMO-GFP fusion protein after incubation with lysed insect cells in the presence of 2% SDS

      Lysates/proteins at 2 µg per lane.
    • ab14405 detecting Smt3 in Escherichia coli recombinant protein (BL21(DE3) by Direct ELISA. Wells were coated with 10µg each of diluted control protein (without SUMO fusion - Negative Controls) and target protein (with SUMO fusion protein) in bicarbonate buffer. Wells were blocked with 5% milk for 1 hour at room temperature. The samples were incubated with primary antibody (1/5000 in TBST +5% milk) for 45 minutes. An HRP-conjugated goat polyclonal to rabbit IgG (1/40000) was used as the secondary antibody.

      See Abreview

    プロトコール

    参考文献

    This product has been referenced in:
    • Neiswinger J  et al. Posttranslational Modification Assays on Functional Protein Microarrays. Cold Spring Harb Protoc 2016:pdb.prot087999 (2016). Read more (PubMed: 27698238) »
    • Bermúdez-López M  et al. Sgs1's roles in DNA end resection, HJ dissolution, and crossover suppression require a two-step SUMO regulation dependent on Smc5/6. Genes Dev 30:1339-56 (2016). Read more (PubMed: 27298337) »

    See all 4 Publications for this product

    レビューと Q&A

    The immunogen is full-length protein, untagged, and the amino acid sequence is given at the UniProt entry for S. cerevisiae Smt3,

    http://www.uniprot.org/uniprot/Q12306 .

    This antibody has not been tested by immunprecipitation, or ...

    Read More
    Abreviews
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (log phase yeast cells)
    Loading amount
    100 µg
    Specification
    log phase yeast cells
    Gel Running Conditions
    Reduced Denaturing (6-12%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 22°C
    Username

    Dr. O Kerscher

    Verified customer

    投稿 Nov 10 2011

    Abreviews
    Application
    ELISA
    Sample
    Escherichia coli Recombinant protein (BL21(DE3))
    Specification
    BL21(DE3)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Type
    Direct
    Username

    Abcam user community

    Verified customer

    投稿 May 28 2010

    Application
    Western blot
    Sample
    Escherichia coli Recombinant protein (BL21(DE3))
    Loading amount
    0.5 µg
    Specification
    BL21(DE3)
    Gel Running Conditions
    Reduced Denaturing (4-20%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Username

    Abcam user community

    Verified customer

    投稿 Apr 26 2010

    Thank you for your enquiry. I am pleased that you are happy with the performance of Smt3 antibody (ab14405). Please can I encourage you to leave an Abreview. This can be done by clicking on the "Abreview" tab on the product datasheet. In return we ...

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    Thank you for your email. We do not routinely offer free or trial sized samples for testing purposes. Our policy at Abcam is that if an antibody does not work as specified on the datasheet, we will offer a replacement or reimbursement. Please rest ...

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    Thank you for your enquiry. Ab5211 is a Fast-Track antibody and has not yet been fully characterized, therefore we cannot guarantee that the antibody will work in any application other than ELISA against the immunizing peptide. We do have some prelimin...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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