Anti-smooth muscle Myosin heavy chain 11 抗体 (ab82541)

製品の概要

  • 製品名Anti-smooth muscle Myosin heavy chain 11 antibody
    smooth muscle Myosin heavy chain 11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to smooth muscle Myosin heavy chain 11
  • アプリケーション適用あり: WB, ICC/IFmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Cow, Orangutan
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 550 - 650 of Human smooth muscle Myosin heavy chain 11.

    (Peptide available as ab93494.)

  • ポジティブ・コントロール
    • This antibody gave a positive signal in the following human lysates: Bladder tissue; Heart tissue; PC3 whole cell; HUVEC whole cell IF/ICC: SV40LT-SMC cell line.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab82541 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use a concentration of 1 µg/ml. Detects a band of approximately 227 kDa (predicted molecular weight: 227 kDa).
ICC/IF Use a concentration of 1 µg/ml.

ターゲット情報

  • 機能Muscle contraction.
  • 組織特異性Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • 関連疾患Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • 配列類似性Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • ドメインThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • 細胞内局在Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

Anti-smooth muscle Myosin heavy chain 11 antibody 画像

  • ICC/IF image of ab82541 stained SV40LT-SMC cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab82541, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • All lanes : Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1 µg/ml

    Lane 1 : PC3 (Human prostate carcinoma cell line) Whole Cell Lysate Whole Cell Lysate
    Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate
    Lane 3 : Human bladder tissue lysate - soluble fraction (female, 45 years) (ab44654)
    Lane 4 : Human heart tissue lysate - total protein (ab29431)

    Lysates/proteins at 10 µg per lane.

    Secondary
    Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
    Developed using the ECL technique

    Performed under reducing conditions.

    Predicted band size : 227 kDa
    Observed band size : 227 kDa
    Additional bands at : 49 kDa,64 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 30 seconds
  • Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) at 1/2000 dilution + whole cell lysate prepared from human PASMC, P6 at 300 µg

    Secondary
    HRP conjugated goat anti-rabbit polyclonal at 1/2000 dilution
    Developed using the ECL technique

    Predicted band size : 227 kDa
    Observed band size : 227 kDa
    Additional bands at : 100 kDa,200 kDa. We are unsure as to the identity of these extra bands.

    Exposure time : 20 seconds

    Image courtesy of an anonymous Abreview.

    See Abreview

Anti-smooth muscle Myosin heavy chain 11 antibody (ab82541) 使用論文

This product has been referenced in:
  • Lian X  et al. Efficient differentiation of human pluripotent stem cells to endothelial progenitors via small-molecule activation of WNT signaling. Stem Cell Reports 3:804-16 (2014). ICC/IF ; Human . Read more (PubMed: 25418725) »

See 1 Publication for this product

Product Wall

Application Immunocytochemistry/ Immunofluorescence
Sample Human Cell (smooth muscle cells derived from hPSCs)
Specification smooth muscle cells derived from hPSCs
Permeabilization No
Fixative Paraformaldehyde
Username

Abcam user community

Verified customer

投稿 Dec 26 2014

Abcam has not validated the combination of species/application used in this Abreview.
Application Western blot
Sample Mouse Tissue lysate - whole (Heart)
Gel Running Conditions Reduced Denaturing (4-12% Bis-Tris Gel)
Loading amount 10 µg
Treatment Day 0 and day 7 post-MI
Specification Heart
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
Username

Dr. Merry Lindsey

Verified customer

投稿 Oct 15 2013

Application Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
Antigen retrieval step Heat mediated - Buffer/Enzyme Used: antigen retrieval
Sample Mouse Tissue sections (Heart at day 0 and day 7 post-MI)
Specification Heart at day 0 and day 7 post-MI
Permeabilization No
Fixative 10% zinc formalin
Username

Dr. Merry Lindsey

Verified customer

投稿 Oct 15 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application Western blot
Sample Human Cell lysate - whole cell (human PASMC, P6)
Loading amount 300 µg
Specification human PASMC, P6
Gel Running Conditions Reduced Denaturing (BioRad Mini-Protean TGX 4-15%)
Blocking step BSA as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Username

Abcam user community

Verified customer

投稿 Aug 08 2011

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"