Anti-smooth muscle Myosin heavy chain 11 抗体 (ab53219)

製品の概要

  • 製品名
    Anti-smooth muscle Myosin heavy chain 11 antibody
    smooth muscle Myosin heavy chain 11 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to smooth muscle Myosin heavy chain 11
  • アプリケーション
    適用あり: WB, IHC-Fr, IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Rat, Sheep, Cow, Human, Pig
    交差が予測される動物種: Vertebrata
  • 免疫原

    Bovine tracheal smooth muscle myosin.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab53219 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB Use at an assay dependent concentration. Predicted molecular weight: 227 kDa.
IHC-Fr Use at an assay dependent concentration.
IHC-P Use at an assay dependent concentration. PubMed: 19729479
ICC/IF Use at an assay dependent concentration.

ターゲット情報

  • 機能
    Muscle contraction.
  • 組織特異性
    Smooth muscle; expressed in the umbilical artery, bladder, esophagus and trachea.
  • 関連疾患
    Note=A chromosomal aberration involving MYH11 is found in acute myeloid leukemia of M4EO subtype. Pericentric inversion inv(16)(p13;q22). The inversion produces a fusion protein consisting of the 165 N-terminal residues of CBF-beta (PEPB2) and the tail region of MYH11.
    Defects in MYH11 are the cause of aortic aneurysm familial thoracic type 4 (AAT4) [MIM:132900]; also known as familial thoracic aortic aneurysm and dissection (TAAD). Aneurysms and dissections of the aorta usually result from degenerative changes in the aortic wall. Thoracic aortic aneurysms and dissections are primarily associated with a characteristic histologic appearance known as 'medial necrosis' or 'Erdheim cystic medial necrosis' in which there is degeneration and fragmentation of elastic fibers, loss of smooth muscle cells, and an accumulation of basophilic ground substance. Patients with AAT4 show marked aortic stiffness. Pathological aortas show large areas of medial degeneration with very low smooth muscle cells content.
  • 配列類似性
    Contains 1 IQ domain.
    Contains 1 myosin head-like domain.
  • ドメイン
    The rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
    Each myosin heavy chain can be split into 1 light meromyosin (LMM) and 1 heavy meromyosin (HMM). It can later be split further into 2 globular subfragments (S1) and 1 rod-shaped subfragment (S2).
  • 細胞内局在
    Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Thick filaments of the myofibrils.
  • Information by UniProt
  • 参照データベース
  • 別名
    • AAT4 antibody
    • DKFZp686D10126 antibody
    • DKFZp686D19237 antibody
    • FAA4 antibody
    • FLJ35232 antibody
    • MGC126726 antibody
    • MGC32963 antibody
    • MYH 11 antibody
    • MYH11 antibody
    • MYH11_HUMAN antibody
    • Myosin 11 antibody
    • Myosin heavy chain 11 antibody
    • Myosin heavy chain 11 smooth muscle antibody
    • Myosin heavy chain antibody
    • Myosin heavy chain smooth muscle isoform antibody
    • Myosin heavy polypeptide 11 smooth muscle antibody
    • Myosin-11 antibody
    • SMHC antibody
    • SMMHC antibody
    • smooth muscle isoform antibody
    • Smooth muscle myosin heavy chain 11 isoform SM2 antibody
    • Smooth muscle myosin heavy chain isoform SM2 antibody
    see all

画像

  • Immuhistochemical analysis of paraffin-embedded sections of uterus labelling smoth muscle myosin chain 11 with ab53219 at 1/500 dilution.

  • ab53219 staining smooth muscle Myosin heavy chain 11 in Pig aortic smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized with 0.1% Triton X-100. Samples were incubated with primary antibody (1/50) for 1 hour at 25°C. Ab6717 (1/400) was used as the secondary antibody.

    See Abreview



  • Predicted band size : 227 kDa
    Observed band size : 228 kDa (why is the actual band size different from the predicted?)

    This image is courtesy of an Abreview submitted by Dr Mario Torrado

    Lanes 3 and 4: Pig Tissue lysate - Heart

    See Abreview

  • ab53219 staining smooth muscle Myosin heavy chain 11 in Human smooth muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and permeabilized and blocked in 1% serum, 0.1%triton, 0.1% BSA in PBS. Samples were incubated with primary antibody (1/100) for 16 hours at 4°C. A Goat anti-rabbit IgG Alexa 488 (green) was used as the secondary antibody, and DAPI was used to stain cell nuclei (blue).

    See Abreview

参考文献

This product has been referenced in:
  • Chan AH  et al. Evaluation of synthetic vascular grafts in a mouse carotid grafting model. PLoS One 12:e0174773 (2017). Read more (PubMed: 28355300) »
  • Huang X  et al. Baicalin attenuates chronic hypoxia-induced pulmonary hypertension via adenosine A2A receptor-induced SDF-1/CXCR4/PI3K/AKT signaling. J Biomed Sci 24:52 (2017). Read more (PubMed: 28774332) »

See all 29 Publications for this product

レビューと Q&A

Application
Flow Cytometry
Sample
Rat Cell (Rat primary smooth muscle cells)
Permeabilization
No
Gating Strategy
Gating were based on two negative controls
Specification
Rat primary smooth muscle cells
Preparation
Cell harvesting/tissue preparation method: Primary rat cells were stained with ab53219 and ab175470 immediately after isolation and run on FACS, around 13% of the cells were positive, which were also what was expected (the remaining cells where fibroblasts, myocytes etc.)
Sample buffer: Stained in 5% FBS-PBS
Fixation
No fixation
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投稿 Jul 10 2017

Application
Western blot
Sample
Rat Tissue lysate - whole (Mesenteric Artery)
Gel Running Conditions
Reduced Denaturing (10% gels)
Loading amount
20 µg
Specification
Mesenteric Artery
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Mr. Edward Humphries

Verified customer

投稿 May 24 2017

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rabbit Tissue sections (Carotid Artery)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10 mM Citrate Buffer (pH=6)
Permeabilization
Yes - 0.1% Triton X-100 for 15 mins
Specification
Carotid Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Paraformaldehyde
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投稿 Oct 20 2016

Application
Western blot
Sample
Mouse Cell lysate - whole cell (aortic smooth muscle cells)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
10 µg
Specification
aortic smooth muscle cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
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Mr. Mc Shen

Verified customer

投稿 May 30 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Xenopus laevis Tissue sections (Heart)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer pH6
Permeabilization
Yes - 1% Triton X100
Specification
Heart
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde
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投稿 Mar 10 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Dog Tissue sections (Uterus)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer pH=6.0
Permeabilization
No
Specification
Uterus
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 10% · Temperature: 20°C
Fixative
Formaldehyde
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投稿 Oct 23 2015

Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (7.5%)
Sample
Rat Cell lysate - whole cell (embryonic rat aortic smooth muscle cell line (A7r5)
Specification
embryonic rat aortic smooth muscle cell line (A7r5
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
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Dr. Cynthia Gershome

Verified customer

投稿 Mar 13 2015

Application
Immunocytochemistry/ Immunofluorescence
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: RT°C
Sample
Human Cell (Human pluripotent stem cell derived cardiomyocyte)
Specification
Human pluripotent stem cell derived cardiomyocyte
Permeabilization
Yes - saponin
Fixative
Paraformaldehyde
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投稿 Nov 19 2014

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Blocking step
1%serum,0.1%triton,0.1%BSA in PBS as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
Sample
Human Cell (smooth muscle cells)
Specification
smooth muscle cells
Permeabilization
Yes - see blocking buffer
Fixative
Paraformaldehyde
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投稿 Jun 07 2013


Myofibroblasts are halfway between differentiation from fibroblasts into smooth muscle cells. Thus, certain markers expressed in SMC could be expected to be expressed in myofibroblasts as well. The paper by Jones et al shows the antibody detects m...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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