Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 1.5% · Temperature: 21°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: antigen retrieval
Sample
Mouse Tissue sections (Heart - day 0 and 7 post-MI)
Specification
Heart - day 0 and 7 post-MI
Permeabilization
No
Fixative
10% zinc formalin
Other product details
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: normal goat serum
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: biotinylated goat anti-rabbit IgG secondary antibo
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Dilution
1/200
Additional data
Additional Notes
Tissue fixed in 10% zinc formalin
Heat mediated antigen retrieval performed using Target Retrieval System, 10X (Dako S1699)
Antibody diluted 1:100 in blocking buffer
No staining identified under these conditions.
Abcam response
We're sorry to hear that ab125884 did not work as stated in the product datasheet. This antibody has been shown to work successfully in IHC-P on human heart and the protocol is provided on the datasheet. Please let us know if results improve when you modify your protocol according to the ab125884 datasheet e.g. fixative, antigen retrieval or staining method.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Merry Lindsey
Verified customer
投稿 Sep 06 2013