製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SMG7
  • 特異性
    This antibody detects endogenous levels of total SMG7 protein.
  • アプリケーション
    適用あり: WB, ICC/IF, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    Synthetic peptide (Human) from an internal region of SMG7.

  • ポジティブ・コントロール
    • WB: Extracts from HeLa cells, Jurkat cells, 293 cells and HUVEC cells. ICC/IF: HepG2 cells.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab64847 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB 1/500 - 1/1000. Detects a band of approximately 126 kDa (predicted molecular weight: 126 kDa).
ICC/IF 1/500 - 1/1000.
ELISA 1/5000.

ターゲット情報

  • 機能
    Plays a role in nonsense-mediated mRNA decay. Recruits UPF1 to cytoplasmic mRNA decay bodies. Together with SMG5 is thought to provide a link to the mRNA degradation machinery involving exonucleolytic pathways, and to serve as an adapter for UPF1 to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation.
  • 配列類似性
    Contains 2 TPR repeats.
  • 細胞内局在
    Cytoplasm. Nucleus. Predominantly cytoplasmic, and nuclear. Shuttles between nucleus and cytoplasm.
  • Information by UniProt
  • 参照データベース
  • 別名
    • breast cancer-associated antigen SGA-56M antibody
    • C1orf16 antibody
    • EST1 like protein C antibody
    • EST1 telomerase component homolog C antibody
    • EST1-like protein C antibody
    • EST1C antibody
    • ever shorter telomeres 1C antibody
    • FLJ23717 antibody
    • hSMG-7 antibody
    • nonsense mediated mRNA decay factor (C. elegans) antibody
    • Protein SMG7 antibody
    • SGA56M antibody
    • SMG 7 antibody
    • SMG-7 homolog antibody
    • Smg7 antibody
    • SMG7_HUMAN antibody
    see all

画像

  • All lanes : Anti-SMG7 antibody (ab64847) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells at 5 µg
    Lane 2 : Extracts of Jurkat cells at 5 µg
    Lane 3 : Extracts of 293 cells at 5 µg
    Lane 4 : Extracts of HUVEC cells at 5 µg
    Lane 5 : Extracts of HUVEC cells plus blocking peptide at 5 µg


    Predicted band size : 126 kDa
    Observed band size : 126 kDa
  • Immunofluorescence analysis of HepG2 cells, using ab64847 (1/500).

    The left panel is in the absence of blocking peptide, whereas the right panel is in the presence of blocking peptide.

参考文献

ab64847 has not yet been referenced specifically in any publications.

レビューと Q&A

Thank you for contacting us. These antibodies have both been tested in a straight ELISA, not in a sandwich ELISA and do not have matched pairs. The detection limit of ab77962 is 1/2,000, for ab64857 it is 1/20,000. Please let me know if you have any fu...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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