製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to SMG5
  • アプリケーション
    適用あり: WBmore details
    適用なし: ICC/IF
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse
  • 免疫原

    Synthetic peptide conjugated to KLH derived from within residues 1000 to the C-terminus of Human SMG5.

    (Peptide available as ab36628.)

  • ポジティブ・コントロール
    • Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー
    Preservative: 0.02% Sodium Azide
    Constituents: 1% BSA, PBS, pH 7.4
  • Concentration information loading...
  • 精製度
    Immunogen affinity purified
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab33033 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
WB
  • 追加情報
    WB: 1/250. Detects a band of approximately 140 kDa (predicted molecular weight: 114 kDa).

    This antibody does not seem suitable to be used for ICC/IF. Please refer to abreviews for further details.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 関連性
      SMG5 plays a role in nonsense mediated mRNA decay. It promotes dephosphorylation of RENT1, and is necessary for TERT activity. It is part of a complex that contains SMG1, SMG5, SMG7, PP2AC, a short isoform of UPF3A (isoform UPF3AS, but not isoform UPF3AL) and phosphorylated RENT1/UPF1.
    • 細胞内局在
      Cytoplasmic and Nuclear
    • 参照データベース
    • 別名
      • EST1 like protein B antibody
      • EST1B antibody
      • LPTS interacting protein antibody
      • LPTS RP1 antibody
      • Nonsense mediated mRNA decay factor antibody
      see all

    画像

    • Anti-SMG5 antibody (ab33033) at 1/250 dilution + Jurkat whole cell lysate (ab7899) at 10 µg

      Secondary
      IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size : 114 kDa
      Observed band size : 140 kDa (why is the actual band size different from the predicted?)
    • IHC image of ab33033 staining in Human breast adenocarcinoma formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab33033, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

      For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    プロトコール

    参考文献

    This product has been referenced in:
    • Park OH  et al. Identification and molecular characterization of cellular factors required for glucocorticoid receptor-mediated mRNA decay. Genes Dev 30:2093-2105 (2016). Read more (PubMed: 27798850) »

    See 1 Publication for this product

    レビューと Q&A

    Abcam has not validated the combination of species/application used in this Abreview.
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (RPE-1)
    Specification
    RPE-1
    Fixative
    Paraformaldehyde
    Permeabilization
    Yes - 0.5% Triton X100 in PBS
    Username

    Dr. Kirk Mcmanus

    Verified customer

    投稿 Sep 04 2007

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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