製品の概要

  • 製品名Anti-Smg1 antibody
    Smg1 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to Smg1
  • アプリケーション適用あり: IHC-Pmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rabbit, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Gorilla, Orangutan, Platypus (Ornithorhynchus anatinus)
  • 免疫原

    Synthetic peptide corresponding to a region between residues 2300 and 2350 of Human Smg1 (Q96QV0)

  • ポジティブ・コントロール
    • Human breast adenocarcinoma tissue

製品の特性

  • 製品の状態Liquid
  • 保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • バッファーPreservative: 0.09% Sodium Azide
    Constituents: 0.1% BSA, Tris buffered saline
  • Concentration information loading...
  • 精製度Immunogen affinity purified
  • 特記事項(精製)Affinity purified using an epitope specific to Smg1 immobilized on solid support.
  • ポリ/モノポリクローナル
  • アイソタイプIgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab82548 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P
  • 追加情報IHC-P: 1/100 - 1/500.
    Epitope exposure with citrate buffer will enhance staining.


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • ターゲット情報

    • 機能Serine/threonine protein kinase involved in both mRNA surveillance and genotoxic stress response pathways. Recognizes the substrate consensus sequence [ST]-Q. Plays a central role in nonsense-mediated decay (NMD) of mRNAs containing premature stop codons by phosphorylating UPF1/RENT1. Recruited by release factors to stalled ribosomes together with SMG8 and SMG9 (forming the SMG1C protein kinase complex), and UPF1 to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Also acts as a genotoxic stress-activated protein kinase that displays some functional overlap with ATM. Can phosphorylate p53/TP53 and is required for optimal p53/TP53 activation after cellular exposure to genotoxic stress. Its depletion leads to spontaneous DNA damage and increased sensitivity to ionizing radiation (IR). May activate PRKCI but not PRKCZ.
    • 組織特異性Widely expressed, with highest level in heart and skeletal muscle. Expressed in placenta, brain, lung and spleen, but not in liver.
    • 配列類似性Belongs to the PI3/PI4-kinase family.
      Contains 1 FAT domain.
      Contains 1 FATC domain.
      Contains 1 HEAT repeat.
      Contains 1 PI3K/PI4K domain.
    • 翻訳後修飾Autophosphorylated.
    • 細胞内局在Nucleus. Cytoplasm.
    • Information by UniProt
    • 参照データベース
    • 別名
      • 61E3.4 antibody
      • ATX antibody
      • hSMG-1 antibody
      • hSMG1 antibody
      • KIAA0421 antibody
      • Lambda interacting protein antibody
      • Lambda-interacting protein antibody
      • Lambda/iota protein kinase C interacting protein antibody
      • Lambda/iota protein kinase C-interacting protein antibody
      • LIP antibody
      • Phosphatidylinositol 3 kinase related kinase antibody
      • Phosphatidylinositol 3 kinase related protein kinase antibody
      • PI 3 kinase related kinase SMG 1 antibody
      • Serine/threonine protein kinase SMG1 antibody
      • Serine/threonine-protein kinase SMG1 antibody
      • SMG-1 antibody
      • smg1 antibody
      • SMG1_HUMAN antibody
      see all

    Anti-Smg1 antibody 画像

    • ab82548, at a 1:250 dilution, staining human Smg1 in breast adenocarcinoma, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue.

    Anti-Smg1 antibody (ab82548) 使用論文

    ab82548 has not yet been referenced specifically in any publications.

    Product Wall

    Thank you for your call today. As we discussed, please forward any protocol information that you can provide and I will be happy to refund this purchase for you. In particular, we would like to know: 1) How was the tissue fixed? ...

    Read More

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"