Anti-SMEK2 抗体 (ab70622)
Key features and details
- Rabbit polyclonal to SMEK2
- Suitable for: IHC-P, WB, IP
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-SMEK2 antibody
SMEK2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to SMEK2 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IPmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Chinese hamster -
免疫原
Synthetic peptide corresponding to Human SMEK2 aa 700-800 (C terminal). Synthetic peptide corresponding to a region between residue 715 and the C-terminus (residue 764) of human SMEK2 (NP_065196.1)
Database link: Q5MIZ7 -
ポジティブ・コントロール
- Whole cell lysates from HeLa, 293T and NIH3T3 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab70622の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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WB |
1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa).
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IP |
Use at 2-5 µg/mg of lysate.
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特記事項 |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
WB
1/2000 - 1/10000. Detects a band of approximately 97 kDa (predicted molecular weight: 97 kDa). |
IP
Use at 2-5 µg/mg of lysate. |
ターゲット情報
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関連性
SMEK2 contains a WH1 domain. There are five named isoforms produced by alternative splicing. Mapping of a diet-induced hypercholesterolemia locus (Dihc2) in rats identified SMEK2 as a strong candidate for responsiveness to dietary cholesterol. -
細胞内局在
Cytoplasm. Cytoskeleton. Centrosome. Nucleus. Note: In interphase localized in the cytoplasm and (with higher levels) the nucleus. During metaphase located in pericentriolar regions. -
参照データベース
- Entrez Gene: 57223 Human
- Entrez Gene: 104570 Mouse
- Omim: 610352 Human
- SwissProt: Q5MIZ7 Human
- SwissProt: Q922R5 Mouse
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別名
- FLFL2 antibody
- FLJ31474 antibody
- KIAA1387 antibody
see all
画像
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling SMEK2 with ab70622 at 1/200 (1µg/ml). Detection: DAB.
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All lanes : Anti-SMEK2 antibody (ab70622) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 97 kDa
Observed band size: 97 kDa -
Immunoprecipitation of HeLa whole cell lysate (1 mg) using ab70622 at 3 µg/ml (lane 1) or control IgG (lane 2). 20% of immunoiprecipitate was analysed by Western blot using ab70622 at 1 µg/ml.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab70622 は 1 報の論文で使用されています。
- Ho MY et al. PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-?B signaling in lung cancer. Oncotarget 7:52255-52269 (2016). PubMed: 27391343